PMID- 11698501
OWN - NLM
STAT- MEDLINE
DCOM- 20011207
LR  - 20161124
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 70
IP  - 5
DP  - 2001 Nov
TI  - Role of Src kinases and Syk in Fcgamma receptor-mediated phagocytosis and 
      phagosome-lysosome fusion.
PG  - 801-11
AB  - Phagocytosis is increased by Fcgamma receptors (FcgammaRs), and studies with 
      syk(-/-) macrophages demonstrated that Syk kinase is required for FcgammaR 
      phagocytosis. Similar studies with macrophages lacking the Src family kinases 
      Hck, Fgr, and Lyn showed that these kinases are not required for phagocytosis but 
      that they enhance the rate of particle engulfment. In this report we show that 
      both wild-type and hck(-/-)fgr(-/-) macrophages expressed Fyn, Src, and Yes and 
      that these kinases were activated on ingestion of immunoglobulin G (IgG)-coated 
      particles and redistributed, together with Syk, to actin-rich phagocytic cups and 
      the phagosomal membrane. At doses blocking IgG-dependent phagocytosis, the 
      tyrosine kinase inhibitors PP1 and piceatannol inhibited both Src family kinase 
      and Syk activities, as well as their redistribution to actin-rich phagocytic 
      cups. Hck, Fgr, and Lyn were dispensable for lysosome-phagosome fusion (PLF) 
      induced by IgG-coated particles. However, PP1 or piceatannol hampered unopsonized 
      yeast-induced PLF despite the fact that they did not block yeast internalization.
FAU - Majeed, M
AU  - Majeed M
AD  - Department of Pathology, Section of General Pathology, University of Verona, 
      Verona, Italy. meyma@ihm.liu.se
FAU - Caveggion, E
AU  - Caveggion E
FAU - Lowell, C A
AU  - Lowell CA
FAU - Berton, G
AU  - Berton G
LA  - eng
GR  - DK50267/DK/NIDDK NIH HHS/United States
GR  - HL54476/HL/NHLBI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (4-amino-5-(4-methylphenyl)-7-(tert-butyl)pyrazolo(3,4-d)pyrimidine)
RN  - 0 (Actins)
RN  - 0 (Biopolymers)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Enzyme Precursors)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Opsonin Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyrimidines)
RN  - 0 (Receptors, IgG)
RN  - 0 (Stilbenes)
RN  - 6KS3LS0D4F (3,3',4,5'-tetrahydroxystilbene)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Fyn protein, mouse)
RN  - EC 2.7.10.2 (Hck protein, mouse)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-fyn)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-hck)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-yes)
RN  - EC 2.7.10.2 (Syk Kinase)
RN  - EC 2.7.10.2 (Syk protein, mouse)
RN  - EC 2.7.10.2 (Yes1 protein, mouse)
RN  - EC 2.7.10.2 (lyn protein-tyrosine kinase)
RN  - EC 2.7.10.2 (proto-oncogene proteins c-fgr)
RN  - EC 2.7.10.2 (src-Family Kinases)
SB  - IM
MH  - Actins/analysis
MH  - Animals
MH  - Biopolymers
MH  - Enzyme Inhibitors/pharmacology
MH  - Enzyme Precursors/antagonists & inhibitors/deficiency/genetics/*physiology
MH  - Immunoglobulin G/immunology
MH  - Intracellular Signaling Peptides and Proteins
MH  - Lysosomes/*physiology
MH  - Membrane Fusion/drug effects
MH  - Mice
MH  - Mice, Knockout
MH  - Microscopy, Confocal
MH  - Microscopy, Fluorescence
MH  - Microspheres
MH  - Opsonin Proteins/immunology
MH  - *Phagocytosis
MH  - Phagosomes/*physiology
MH  - Protein-Tyrosine Kinases/antagonists & inhibitors/deficiency/genetics/*physiology
MH  - Proto-Oncogene Proteins/antagonists & inhibitors/deficiency/genetics/physiology
MH  - Proto-Oncogene Proteins c-fyn
MH  - Proto-Oncogene Proteins c-hck
MH  - Proto-Oncogene Proteins c-yes
MH  - Pyrazoles/pharmacology
MH  - Pyrimidines/pharmacology
MH  - Receptors, IgG/*physiology
MH  - Saccharomyces cerevisiae
MH  - Stilbenes/pharmacology
MH  - Syk Kinase
MH  - src-Family Kinases/antagonists & inhibitors/deficiency/genetics/*physiology
EDAT- 2001/11/08 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/08 10:00
PHST- 2001/11/08 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/08 10:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2001 Nov;70(5):801-11.