PMID- 11700861
OWN - NLM
STAT- MEDLINE
DCOM- 20020502
LR  - 20191105
IS  - 0196-3635 (Print)
IS  - 0196-3635 (Linking)
VI  - 22
IP  - 6
DP  - 2001 Nov-Dec
TI  - Fluorescence in situ hybridization analysis of sex-chromosome mosaicism in 
      azoospermic men.
PG  - 970-2
AB  - A retrospective study was carried out to determine the prevalence of 
      sex-chromosome mosaicism among azoospermic men and to evaluate the feasibility of 
      using fluorescence in situ hybridization (FISH) technique to assess mosaicism and 
      the origin of marker chromosomes. Nine hundred eighty patients with azoospermia 
      who were referred to a male infertility clinic at a university hospital were 
      karyotyped by G-banding using peripheral blood lymphocyte (PBL) metaphase 
      spreads. When sex chromosome mosaic karyotype was detected, FISH analyses using 
      sex chromosome-specific probes were performed. Seventeen of 980 patients showed 
      evidence of sex chromosomal mosaic karyotype or mosaicism with marker chromosomes 
      by G-banding studies of PBLs. Ten patients showed mosaicism in the number of sex 
      chromosomes and 7 showed mosaicism with marker chromosomes. All 17 patients 
      agreed to undergo FISH analysis. FISH confirmed mosaicism in 88.2% of these 
      patients (15 of 17). Low-frequency mosaicism showing a frequency of less than 10% 
      by G-banding was proved to be nonmosaicism by FISH. Marker chromosomes detected 
      in 7 patients were proved to be derived from the Y chromosome by FISH analyses. 
      From these data the prevalence of sex chromosome mosaicism confirmed by FISH 
      analysis is 1.5% (15 of 980 patients). FISH analysis should be applied when 
      mosaicism shows a frequency of less than 10% by the G-banding technique. Also, 
      FISH analysis is indicated when a marker chromosome is detected by G-banding.
FAU - Okada, H
AU  - Okada H
AD  - Department of Organ Therapeutics, Faculty of Medicine, Kobe University Graduate 
      School of Medicine, Japan. okada@med.kobe-u.ac.jp
FAU - Dobashi, M
AU  - Dobashi M
FAU - Yamazaki, T
AU  - Yamazaki T
FAU - Fujisawa, M
AU  - Fujisawa M
FAU - Arakawa, S
AU  - Arakawa S
FAU - Kamidono, S
AU  - Kamidono S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
SB  - IM
MH  - Animals
MH  - *Chromosome Aberrations
MH  - Chromosome Banding
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - *Mosaicism
MH  - Oligospermia/*genetics
MH  - *Sex Chromosomes
EDAT- 2001/11/10 10:00
MHDA- 2002/05/03 10:01
CRDT- 2001/11/10 10:00
PHST- 2001/11/10 10:00 [pubmed]
PHST- 2002/05/03 10:01 [medline]
PHST- 2001/11/10 10:00 [entrez]
AID - 10.1002/j.1939-4640.2001.tb03437.x [doi]
PST - ppublish
SO  - J Androl. 2001 Nov-Dec;22(6):970-2. doi: 10.1002/j.1939-4640.2001.tb03437.x.