PMID- 11702662
OWN - NLM
STAT- MEDLINE
DCOM- 20021030
LR  - 20061115
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 81
IP  - 15
DP  - 2001 Aug 10
TI  - [Significance of her2 oncogene expression in primary nasopharyngeal carcinoma].
PG  - 904-6
AB  - OBJECTIVE: To investigate the status of her2 oncogene at DNA level and the 
      molecular mechanism of overexpression of her2 oncoprotein in part of patients 
      with nasopharyngeal carcinoma (NPC). METHODS: Forty-five cases of PNC tissue and 
      fifteen cases of normal epithelial tissue adjacent to tumor tissue were examined 
      for her 2 signal and centromere 17 signal. Interphase fluorescence in situ 
      hybridization (FISH) was used to examine the copy number of her 2 and 
      immunohistochemical (IHC) staining were used to analyze the copy number and 
      protein expression of her2. RESULTS: There were 1.92% +/- 0.10% her2 signals per 
      nucleus in normal epithelial tissue adjacent to tumor. 75.52 +/- 9.33% of the 
      nuclei contained 2 her2 signals and 2 centromere 17 signals. No alteration in 
      gene copy number was found in cancerous nuclei. However, IHC staining of the 45 
      tumors with antibody against her2 showed that overexpression of her2 oncoprotein 
      occurred in 33% (15/45) of NPC. However, there was no significant correlation 
      between her2 expression and sex, age, clinical staging, and clinical outcome of 
      patients. CONCLUSION: The overexpression of her2 in NPC does not relate to her2 
      gene amplification. Immunohistochemistry may not be a good measure to examine 
      her2 expression. The mechanism of her2 oncoprotein overexpression and its 
      biological significance in the development of NPC remain to be elucidated.
FAU - Yan, J
AU  - Yan J
AD  - Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, 
      China.
FAU - Fang, Y
AU  - Fang Y
FAU - Jiao, Y
AU  - Jiao Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Female
MH  - Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Nasopharyngeal Neoplasms/*genetics/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/*biosynthesis/genetics
MH  - RNA, Messenger/analysis
EDAT- 2001/11/13 10:00
MHDA- 2002/10/31 04:00
CRDT- 2001/11/13 10:00
PHST- 2001/11/13 10:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2001/11/13 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2001 Aug 10;81(15):904-6.