PMID- 1170278
OWN - NLM
STAT- MEDLINE
DCOM- 19750913
LR  - 20061115
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 27
IP  - 3
DP  - 1975 Jun
TI  - Physico-chemical and serological characterization of five rhabdoviruses infecting 
      fish.
PG  - 369-78
AB  - Viruses isolated from fish with viral haemorrhagic septicaemia (VHS), infectious 
      haematopoietic necrosis (IHN), spring viraemia of carp (SVC), swim-bladder 
      inflammation (SBI) and pike fry disease (PFD) have been grown to high titre in 
      fathead minnow cells. While our preparations of the IHN, SVC, SBI and PFD viruses 
      showed typical rhabdovirus morphology with bullet-shaped particles and distinct 
      surface projections, the VHS virus preparations had a less typical rhabdovirus 
      morphology but were pleomorphic with a preponderance of flexuous rods. Using 
      virus labelled with [-3H]-uridine, it was shown that each virus contained RNA 
      which sedimented at 38 to 40 S and was hydrolysed by very low concentrations of 
      ribonuclease. The viruses of SVC, PFD and SBI had a polypeptide composition 
      similar to that of vesicular stomatitis virus, the prototype rhabdovirus, but the 
      IHN and VHS viruses gave a pattern similar to that of rabies virus. In serum 
      neutralization tests the SVC and SBI viruses were indistinguishable. VHS virus 
      showed no serological relationship with the other four viruses but there was a 
      low level of cross-reaction between the PFD, IHN and SVC-SBI viruses.
FAU - Hill, B J
AU  - Hill BJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - 0 (Antigens, Viral)
RN  - 0 (Peptides)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antigens, Viral/analysis
MH  - Cell Line
MH  - Centrifugation, Density Gradient
MH  - Cyprinidae
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Epithelial Cells
MH  - Fish Diseases/*microbiology
MH  - Microscopy, Electron
MH  - Neutralization Tests
MH  - Peptides/analysis
MH  - *RNA Viruses/analysis/immunology/ultrastructure
MH  - RNA, Viral/analysis
MH  - Viral Proteins/analysis
MH  - Virus Cultivation
EDAT- 1975/06/01 00:00
MHDA- 1975/06/01 00:01
CRDT- 1975/06/01 00:00
PHST- 1975/06/01 00:00 [pubmed]
PHST- 1975/06/01 00:01 [medline]
PHST- 1975/06/01 00:00 [entrez]
AID - 10.1099/0022-1317-27-3-369 [doi]
PST - ppublish
SO  - J Gen Virol. 1975 Jun;27(3):369-78. doi: 10.1099/0022-1317-27-3-369.