PMID- 11708491
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20190616
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 945
DP  - 2001 Sep
TI  - Possible origin of extrachromosomal DNA from human lymphocytes following in vitro 
      treatment by phytohemagglutinin.
PG  - 289-91
AB  - Lymphocytes respond to chemical or pathological challenges by synthesizing 
      episomes or extrachromosomal DNA (eDNA). Phytohemagglutinin (PHA) is a mitogen 
      for T lymphocytes and can stimulate the increased production of episomes as well 
      as the release of eDNA, interleukin-2, and other factors. However, the origin of 
      this eDNA is largely unknown. To confirm the observation on the localization of 
      an antibody directed against a 23-kb segment of bull acrosomal DNA in cultured 
      human lymphocytes, we performed analyses by fluorescence in situ hybridization 
      (FISH) using the same DNA as a molecular probe.
FAU - Chen, J S
AU  - Chen JS
AD  - Centro per lo Studio delle Cellule Germinale, Consiglio Nazionale delle Ricerche, 
      Siena, Italy. chen@unisi.it
FAU - Chen, M G
AU  - Chen MG
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Phytohemagglutinins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cells, Cultured
MH  - DNA/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*drug effects/metabolism
MH  - Phytohemagglutinins/*pharmacology
EDAT- 2001/11/16 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/16 10:00
PHST- 2001/11/16 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/16 10:00 [entrez]
AID - 10.1111/j.1749-6632.2001.tb03897.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2001 Sep;945:289-91. doi: 10.1111/j.1749-6632.2001.tb03897.x.