PMID- 11709288
OWN - NLM
STAT- MEDLINE
DCOM- 20020723
LR  - 20191105
IS  - 1286-4579 (Print)
IS  - 1286-4579 (Linking)
VI  - 3
IP  - 13
DP  - 2001 Nov
TI  - Spontaneous reduction in Epstein-Barr virus (EBV) DNA copy number in EBV-infected 
      epithelial cell lines.
PG  - 1085-91
AB  - We found that spontaneous and 12-0-tetradecanoylphorbol-13-acetate-induced 
      Epstein-Barr virus (EBV) reactivation occurred in short-term (ST)-cultured 
      EBV-infected epithelial cell lines GT38 and GT39 after their establishment; 
      however, it diminished in the long-term (LT)-cultured cells passaged for more 
      than 2 years from ST-cultured cells. We hypothesized that the EBV reactivation 
      may be related to the EBV DNA copy number in the cells. A higher level of EBV DNA 
      content was detected in ST-cultured cells than in LT-cultured cells by Southern 
      hybridization using an EBV DNA XhoI probe. Fluorescence in situ hybridization 
      using EBV DNA BamHI W fragments showed that ST-cultured cells contained a higher 
      EBV DNA copy number than that of LT-cultured cells. EBV DNA-negative cells were 
      detected in small proportions in LT-cultured cells, but were undetected in 
      ST-cultured cells. These results demonstrate that EBV genomes are not maintained 
      stably in the cell lines, and some of them are lost in continuous passages of the 
      cells. We discuss the mechanisms of reduction of EBV reactivation and EBV DNA in 
      the cell lines.
FAU - Kanamori, M
AU  - Kanamori M
AD  - Department of Biosignaling, School of Life Science, Faculty of Medicine, Tottori 
      University, 683-8503, Yonago, Japan.
FAU - Murakami, M
AU  - Murakami M
FAU - Takahashi, T
AU  - Takahashi T
FAU - Kamada, N
AU  - Kamada N
FAU - Tajima, M
AU  - Tajima M
FAU - Okinaga, K
AU  - Okinaga K
FAU - Miyazawa, Y
AU  - Miyazawa Y
FAU - Kurata, T
AU  - Kurata T
FAU - Sairenji, T
AU  - Sairenji T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Blotting, Western
MH  - Cell Line, Transformed
MH  - DNA Replication
MH  - DNA, Viral/*biosynthesis/genetics
MH  - Epithelial Cells/ultrastructure/*virology
MH  - Fluorescent Antibody Technique
MH  - Gene Dosage
MH  - Herpesvirus 4, Human/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Time Factors
MH  - Virus Activation
MH  - *Virus Replication
EDAT- 2001/11/16 10:00
MHDA- 2002/07/24 10:01
CRDT- 2001/11/16 10:00
PHST- 2001/11/16 10:00 [pubmed]
PHST- 2002/07/24 10:01 [medline]
PHST- 2001/11/16 10:00 [entrez]
AID - S1286-4579(01)01468-X [pii]
AID - 10.1016/s1286-4579(01)01468-x [doi]
PST - ppublish
SO  - Microbes Infect. 2001 Nov;3(13):1085-91. doi: 10.1016/s1286-4579(01)01468-x.