PMID- 11709514
OWN - NLM
STAT- MEDLINE
DCOM- 20011228
LR  - 20220316
IS  - 0017-5749 (Print)
IS  - 1458-3288 (Electronic)
IS  - 0017-5749 (Linking)
VI  - 49
IP  - 6
DP  - 2001 Dec
TI  - Interleukin 16 expression and phenotype of interleukin 16 producing cells in 
      Crohn's disease.
PG  - 795-803
AB  - BACKGROUND: The mechanisms involved in the initiation and maintenance of Crohn's 
      disease are poorly understood. Previous studies have demonstrated an increased 
      number of infiltrating CD4+ T cells within the inflammatory affected bowel wall 
      in Crohn's disease. Novel therapy approaches using anti-CD4 antibodies are 
      thought to be effective in Crohn's disease. AIMS: Interleukin 16 (IL-16) has been 
      characterised as a chemokine with selective chemoattraction for CD4+ inflammatory 
      T cells. In this study, cellular expression of IL-16 in Crohn's disease and 
      ulcerative colitis was investigated. METHODS: Expression of IL-16 was analysed in 
      tissue samples of Crohn's disease, ulcerative colitis, and normal controls by 
      applying reverse transcription-polymerase chain reaction, non-radioactive in situ 
      hybridisation, and immunohistochemistry. Double staining methods were used to 
      characterise cells expressing IL-16. The amount of infiltrating CD4+ cells was 
      determined by immunohistochemistry and correlated with the corresponding IL-16+ 
      cell number by step sections. RESULTS: An increased number of IL-16+ cells in 
      Crohn's disease in comparison with ulcerative colitis and control probes was 
      demonstrated. IL-16 was expressed by CD4 and CD8 positive T cells. In addition, 
      in active Crohn's disease there was a substantial number of IL-16 positive mast 
      cells. The increased number of CD4+ lymphocytes correlated positively with the 
      increased number of IL-16 positive cells in Crohn's disease. CONCLUSION: Our 
      results demonstrate that increased expression of IL-16 in T cells and mast cells 
      in active Crohn's disease is associated with increased numbers of CD4+ 
      lymphocytes. Local expression of IL-16 seems to play a significant role in the 
      initiation and persistence of the inflammatory process in Crohn's disease, 
      presumably by IL-16 mediated recruitment of CD4+ cells, mostly lymphocytes, into 
      the bowel wall.
FAU - Middel, P
AU  - Middel P
AD  - Department of Pathology, Georg-August University of Gottingen, Germany. 
      pmiddel@med.uni-goettingen.de
FAU - Reich, K
AU  - Reich K
FAU - Polzien, F
AU  - Polzien F
FAU - Blaschke, V
AU  - Blaschke V
FAU - Hemmerlein, B
AU  - Hemmerlein B
FAU - Herms, J
AU  - Herms J
FAU - Korabiowska, M
AU  - Korabiowska M
FAU - Radzun, H J
AU  - Radzun HJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Gut
JT  - Gut
JID - 2985108R
RN  - 0 (Interleukin-16)
SB  - IM
CIN - Gut. 2001 Dec;49(6):747-8. PMID: 11709502
MH  - Adolescent
MH  - Adult
MH  - CD4 Lymphocyte Count
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Chemotaxis, Leukocyte
MH  - Colitis, Ulcerative/immunology
MH  - Colon/immunology
MH  - Crohn Disease/*immunology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization/methods
MH  - Interleukin-16/*analysis
MH  - Male
MH  - Mast Cells/immunology
MH  - Middle Aged
MH  - Phenotype
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - T-Lymphocytes/*immunology
PMC - PMC1728558
EDAT- 2001/11/16 10:00
MHDA- 2002/01/05 10:01
PMCR- 2004/12/01
CRDT- 2001/11/16 10:00
PHST- 2001/11/16 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/16 10:00 [entrez]
PHST- 2004/12/01 00:00 [pmc-release]
AID - 10.1136/gut.49.6.795 [doi]
PST - ppublish
SO  - Gut. 2001 Dec;49(6):795-803. doi: 10.1136/gut.49.6.795.