PMID- 11710680
OWN - NLM
STAT- MEDLINE
DCOM- 20011205
LR  - 20081121
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 116
IP  - 5
DP  - 2001 Nov
TI  - Prevalence and cellular reservoir of latent human herpesvirus 6 in tonsillar 
      lymphoid tissue.
PG  - 648-54
AB  - There are few studies that examine prevalence, quantity, and cellular proclivity 
      of latent human herpesvirus 6 (HHV-6) in healthy populations. We examined 69 
      tonsils with paired blood specimens from children without evidence of acute 
      infection. By polymerase chain reaction (PCR), HHV-6 was detected at low levels 
      in 100% of tonsils and 39% of blood samples (n = 27), suggesting that prevalence 
      of latent HHV-6 infection is high in children and may be underestimated by PCR 
      analysis of blood. Although HHV-6A and HHV-6B were detected, HHV-6B predominated, 
      being found in 97% of samples (n = 67). Tonsil sections from 7 cases were 
      examined by in situ hybridization using 2 HHV-6 probes and immunohistochemical 
      analysis. Using both in situ hybridization and immunohistochemical analysis, all 
      tissues revealed marked HHV-6-specific staining in the squamous epithelium of the 
      tonsillar crypts and rare positive lymphocytes. We conclude that HHV-6 is present 
      universally in tonsils of children, and tonsillar epithelium may be an important 
      viral reservoir in latent infection.
FAU - Roush, K S
AU  - Roush KS
AD  - Department of Pathology, University of Texas Southwestern Medical Center at 
      Dallas, USA.
FAU - Domiati-Saad, R K
AU  - Domiati-Saad RK
FAU - Margraf, L R
AU  - Margraf LR
FAU - Krisher, K
AU  - Krisher K
FAU - Scheuermann, R H
AU  - Scheuermann RH
FAU - Rogers, B B
AU  - Rogers BB
FAU - Dawson, D B
AU  - Dawson DB
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Child, Preschool
MH  - DNA Primers/chemistry
MH  - DNA, Viral/analysis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Exanthema Subitum/pathology/*virology
MH  - Female
MH  - Herpesvirus 6, Human/classification/genetics/*isolation & purification
MH  - Humans
MH  - In Situ Hybridization
MH  - Infant
MH  - Lymphocytes/pathology/virology
MH  - Male
MH  - Palatine Tonsil/pathology/*virology
MH  - Polymerase Chain Reaction
EDAT- 2001/11/17 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/17 10:00
PHST- 2001/11/17 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/17 10:00 [entrez]
AID - 10.1309/Y2HH-B1CK-0F5L-U7B8 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2001 Nov;116(5):648-54. doi: 10.1309/Y2HH-B1CK-0F5L-U7B8.