PMID- 11710860
OWN - NLM
STAT- MEDLINE
DCOM- 20011220
LR  - 20191105
IS  - 1521-2491 (Print)
IS  - 1521-2491 (Linking)
VI  - 3
IP  - 4
DP  - 2001 Oct-Dec
TI  - Effect of blended carbon dioxide and erbium:YAG laser energy on preauricular and 
      ear lobule keloid fibroblast secretion of growth factors: a serum-free study.
PG  - 252-7
AB  - BACKGROUND: A serum-free in vitro model was used to determine the effect of 
      combined carbon dioxide (CO2) and erbium (Er):YAG laser (Derma K; ESC/Sharplan 
      Medical Systems, Yokneam, Israel) irradiation on keloid-producing fibroblasts 
      (KFs) from 2 distinct facial sites. Transforming growth factor beta1 (TGF-beta1) 
      and basic fibroblast growth factor (bFGF) play an integral part in wound healing 
      and were assayed using this model. It has always been a clinical impression that 
      fibroblasts from different regions of the face behave differently. This is 
      exemplified by patients prone to lobule keloid formation after ear piercing, who 
      heal normally after a facial incision. DESIGN: Laboratory-based wound healing. 
      METHODS: Human KF cell lines were established from operative specimens using 
      standard explant techniques. At 48 hours after seeding, 20% of each well was 
      exposed to 1.7 J/pulse of Er:YAG laser energy and CO2 delivered at 3 or 5 W and 
      at a duty cycle of 25%, 50%, or 100%. Using a quantitative enzyme-linked 
      immunosorbent assay, TGF-beta1 and bFGF were assayed from collected supernatants. 
      RESULTS: Laser-treated ear lobule KFs demonstrated decreased TGF-beta1 production 
      when compared with preauricular KFs. Statistical significance (P<.005) was seen 
      in the 3-W CO2 25% duty cycle; a trend was seen in the 3-W CO2 50% duty cycle 
      (P<.08). Preauricular KFs secreted increased bFGF when compared with lobule KFs. 
      Significance was seen in the 3-W CO2 25% and 50% duty cycles (P<.05). 
      Laser-treated preauricular KFs had increased bFGF secretion when compared with 
      non-laser-treated preauricular KFs in the 3-W CO2 25%, 50%, and 100% duty cycles. 
      CONCLUSIONS: Combined CO2 and Er:YAG laser treatment decreases the production of 
      TGF-beta1 in preauricular and ear lobule KFs. This laser may have clinical 
      promise in the treatment of keloids. Finally, the different growth factor 
      profiles obtained suggest that KFs from the ear lobule and preauricular regions 
      are different.
FAU - Cheng, E T
AU  - Cheng ET
AD  - Wound Healing and Tissue Engineering Laboratory, Division of Otolaryngology-Head 
      and Neck Surgery, R-135, Stanford University Medical Center, Stanford, CA 
      94305-5328, USA.
FAU - Nowak, K C
AU  - Nowak KC
FAU - Koch, R J
AU  - Koch RJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Arch Facial Plast Surg
JT  - Archives of facial plastic surgery
JID - 100883500
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 142M471B3J (Carbon Dioxide)
RN  - 77B218D3YE (Erbium)
SB  - IM
MH  - Carbon Dioxide
MH  - Cells, Cultured
MH  - Culture Media, Serum-Free
MH  - *Ear, External
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Erbium
MH  - Fibroblast Growth Factor 2/*biosynthesis
MH  - Fibroblasts/*metabolism/radiation effects
MH  - Humans
MH  - Keloid/*metabolism/radiotherapy
MH  - *Lasers
MH  - Transforming Growth Factor beta/*biosynthesis
MH  - Transforming Growth Factor beta1
EDAT- 2001/11/17 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/17 10:00
PHST- 2001/11/17 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/17 10:00 [entrez]
AID - qoa00039 [pii]
AID - 10.1001/archfaci.3.4.252 [doi]
PST - ppublish
SO  - Arch Facial Plast Surg. 2001 Oct-Dec;3(4):252-7. doi: 10.1001/archfaci.3.4.252.