PMID- 11711433
OWN - NLM
STAT- MEDLINE
DCOM- 20011218
LR  - 20220129
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 15
IP  - 22
DP  - 2001 Nov 15
TI  - FHY1: a phytochrome A-specific signal transducer.
PG  - 2980-90
AB  - Phytochromes are plant photoreceptors that regulate plant growth and development 
      with respect to the light environment. Following the initial light-perception 
      event, the phytochromes initiate a signal-transduction process that eventually 
      results in alterations in cellular behavior, including gene expression. Here we 
      describe the molecular cloning and functional characterization of Arabidopsis 
      FHY1. FHY1 encodes a product (FHY1) that specifically transduces signals 
      downstream of the far-red (FR) light-responsive phytochrome A (PHYA) 
      photoreceptor. We show that FHY1 is a novel light-regulated protein that 
      accumulates in dark (D)-grown but not in FR-grown hypocotyl cells. In addition, 
      FHY1 transcript levels are regulated by light, and by the product of FHY3, 
      another gene implicated in FR signaling. These observations indicate that FHY1 
      function is both FR-signal transducing and FR-signal regulated, suggesting a 
      negative feedback regulation of FHY1 function. Seedlings homozygous for 
      loss-of-function fhy1 alleles are partially blind to FR, whereas seedlings 
      overexpressing FHY1 exhibit increased responses to FR, but not to white (WL) or 
      red (R) light. The increased FR-responses conferred by overexpression of FHY1 are 
      abolished in a PHYA-deficient mutant background, showing that FHY1 requires a 
      signal from PHYA for function, and cannot modulate growth independently of PHYA.
FAU - Desnos, T
AU  - Desnos T
AD  - John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
FAU - Puente, P
AU  - Puente P
FAU - Whitelam, G C
AU  - Whitelam GC
FAU - Harberd, N P
AU  - Harberd NP
LA  - eng
GR  - BBS/E/J/00000583/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (FHY1 protein, Arabidopsis)
RN  - 0 (Luminescent Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 11121-56-5 (Phytochrome)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Arabidopsis/genetics
MH  - Arabidopsis Proteins/*genetics/*metabolism
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - Contig Mapping
MH  - Glutathione Transferase/metabolism
MH  - Green Fluorescent Proteins
MH  - Introns
MH  - Light
MH  - Luminescent Proteins/metabolism
MH  - Models, Genetic
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phytochrome/*genetics/*metabolism/*physiology
MH  - Plants, Genetically Modified/genetics
MH  - Protein Binding
MH  - RNA, Messenger/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sequence Homology, Amino Acid
MH  - *Signal Transduction
PMC - PMC312831
EDAT- 2001/11/17 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/05/15
CRDT- 2001/11/17 10:00
PHST- 2001/11/17 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/17 10:00 [entrez]
PHST- 2002/05/15 00:00 [pmc-release]
AID - 10.1101/gad.205401 [doi]
PST - ppublish
SO  - Genes Dev. 2001 Nov 15;15(22):2980-90. doi: 10.1101/gad.205401.