PMID- 11712086
OWN - NLM
STAT- MEDLINE
DCOM- 20020306
LR  - 20071115
IS  - 1107-3756 (Print)
IS  - 1107-3756 (Linking)
VI  - 8
IP  - 6
DP  - 2001 Dec
TI  - Differential expression of multiple alternative spliceforms of the Men1 tumor 
      suppressor gene in mouse.
PG  - 681-9
AB  - The multiple endocrine neoplasia type 1 gene (MEN1) is a tumor suppressor gene 
      associated with the development of tumors in the parathyroids, the pituitary, and 
      the pancreas and has also been linked to impaired germ cell production. The 
      murine ortholog, Men1, is highly homologous to the human counterpart both at DNA 
      and protein levels. The present study was undertaken to further approach the 
      function of Men1 and its encoded protein menin. By 5' RACE and RT-PCR four 
      alternative splice variants were identified, indicating a 5' heterogeneity of 
      Men1 similar to the human counterpart. By mRNA in situ hybridization of embryonal 
      and adult mouse tissues, all four splice variants were shown to be expressed, 
      albeit at varying timepoints and levels in the different tissues. However, a 
      putative isoform postulated from the DNA sequence, which would elongate the 
      reading frame by 15 bases at the exon 2/intron 2 junction, was not found to occur 
      in mouse. The strongest expression was detected in testis, both at the mRNA and 
      protein level and was therefore further characterized by protein analysis of 
      cells isolated from different stages of the spermatogenesis. Western blotting 
      revealed a single protein of approximately 70 kDa detected in total testis, 
      isolated pachytene spermatocytes and in haploid spermatids. Notably, no menin 
      expression was detectable in the extracts from epididymis where the maturation of 
      sperms is almost completed, suggesting that menin plays a crucial role during 
      spermatogenesis.
FAU - Forsberg, L
AU  - Forsberg L
AD  - Department of Molecular Medicine, Endocrine tumor unit, Karolinska Hospital CMM 
      L8:01, SE-171 76 Stockholm, Sweden. lars.forsberg@cmm.ki.se
FAU - Zablewska, B
AU  - Zablewska B
FAU - Piehl, F
AU  - Piehl F
FAU - Weber, G
AU  - Weber G
FAU - Lagercrantz, S
AU  - Lagercrantz S
FAU - Gaudray, P
AU  - Gaudray P
FAU - Hoog, C
AU  - Hoog C
FAU - Larsson, C
AU  - Larsson C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Int J Mol Med
JT  - International journal of molecular medicine
JID - 9810955
RN  - 0 (MEN1 protein, human)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Alternative Splicing
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Embryo, Mammalian/metabolism
MH  - Female
MH  - Gene Expression Regulation
MH  - Gene Expression Regulation, Developmental
MH  - Genes, Tumor Suppressor
MH  - In Situ Hybridization
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Neoplasm Proteins/*genetics/metabolism
MH  - Pregnancy
MH  - Protein Isoforms/genetics/metabolism
MH  - *Proto-Oncogene Proteins
MH  - RNA, Messenger/genetics/metabolism
MH  - Spermatogenesis/genetics
MH  - Tissue Distribution
EDAT- 2001/11/17 10:00
MHDA- 2002/03/07 10:01
CRDT- 2001/11/17 10:00
PHST- 2001/11/17 10:00 [pubmed]
PHST- 2002/03/07 10:01 [medline]
PHST- 2001/11/17 10:00 [entrez]
PST - ppublish
SO  - Int J Mol Med. 2001 Dec;8(6):681-9.