PMID- 11712764
OWN - NLM
STAT- MEDLINE
DCOM- 20020404
LR  - 20190921
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 46
IP  - 5
DP  - 2001 Nov
TI  - Expression of interleukin-18 and its receptor in mouse ovary.
PG  - 349-57
AB  - PROBLEM: Interleukin-18 (IL-18) strongly induces interferon-gamma production and 
      is produced not only by types of immune cells but also by types of non-immune 
      cells. Ovulation is thought to be an inflammation-like reaction in which many 
      pro-inflammatory cytokines are involved. We investigated whether IL-18 is 
      involved in the functions of ovary. METHOD OF STUDY: The 4-week-old immature 
      female mice were examined for IL-18 and IL-18 receptor (IL-18R) expression on 
      their ovaries under stimulation with pregnant mare's serum gonadotropin (PMSG) 
      and human chorionic gonadotropin (hCG) by immunohistochemical staining, Western 
      blotting and reverse transcript-polymerase chain reaction. The IL-18R was blocked 
      by the injection of anti-IL-18R monoclonal antibody to immature mice during 
      PMSG-hCG stimulation, and the number of ovulated ova was counted. RESULTS: The 
      expression of both proteins and mRNA of IL-18 and IL-18R were very low in 
      immature ovaries before stimulation, but after PMSG injection both IL-18 and 
      IL-18R increased dramatically in theca cells and reached a maximum level at the 
      peri-ovulatory phase then slightly lowered, but still kept a high level during 
      the luteal phase in the corpus luteum. The treatment of IL-18R monoclonal 
      antibody to the mice during ovarian stimulation reduced the number of ovulated 
      ova and inhibited the expansion of cumulus cells surrounding the ovum. 
      CONCLUSION: IL-18 and IL-18R play roles in various kinds of function of ovary.
FAU - Tsuji, Y
AU  - Tsuji Y
AD  - Department of Obstetrics and Gynecology, Hyogo College of Medicine, Japan. 
      yoshi416@hyo-med.ac.jp
FAU - Tamaoki, T H
AU  - Tamaoki TH
FAU - Hasegawa, A
AU  - Hasegawa A
FAU - Kashiwamura, S
AU  - Kashiwamura S
FAU - Iemoto, A
AU  - Iemoto A
FAU - Ueda, H
AU  - Ueda H
FAU - Muranaka, J
AU  - Muranaka J
FAU - Adachi, S
AU  - Adachi S
FAU - Furuyama, J
AU  - Furuyama J
FAU - Okamura, H
AU  - Okamura H
FAU - Koyama, K
AU  - Koyama K
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (Gonadotropins, Equine)
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Il18r1 protein, mouse)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Chorionic Gonadotropin/pharmacology
MH  - Female
MH  - Gonadotropins, Equine/pharmacology
MH  - Immunohistochemistry
MH  - Interleukin-18/*analysis/genetics/physiology
MH  - Interleukin-18 Receptor alpha Subunit
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Molecular Weight
MH  - Ovary/*chemistry
MH  - Ovulation
MH  - Receptors, Interleukin/*analysis/genetics/physiology
MH  - Receptors, Interleukin-18
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/11/20 10:00
MHDA- 2002/04/18 10:01
CRDT- 2001/11/20 10:00
PHST- 2001/11/20 10:00 [pubmed]
PHST- 2002/04/18 10:01 [medline]
PHST- 2001/11/20 10:00 [entrez]
AID - 10.1034/j.1600-0897.2001.d01-23.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2001 Nov;46(5):349-57. doi: 
      10.1034/j.1600-0897.2001.d01-23.x.