PMID- 11713345
OWN - NLM
STAT- MEDLINE
DCOM- 20020111
LR  - 20071115
IS  - 1066-5099 (Print)
IS  - 1066-5099 (Linking)
VI  - 19
IP  - 6
DP  - 2001
TI  - Prenatal diagnosis of genetic abnormalities using fetal CD34+ stem cells in 
      maternal circulation and evidence they do not affect diagnosis in later 
      pregnancies.
PG  - 534-42
AB  - In the present study, we report a new method for enrichment and analysis of fetal 
      CD34+ stem cells after culture in order to determine whether it is feasible for 
      noninvasive prenatal diagnosis. We also determined whether fetal CD34+ stem cells 
      persist in maternal blood after delivery and assessed whether they have an impact 
      on noninvasive prenatal diagnosis of genetic abnormalities. Peripheral blood 
      samples were obtained from 35 pregnant women, 13 non-pregnant women who had given 
      birth to male offsprings, 12 women who had never been pregnant, and eight 
      pregnant women with male fetuses. CD34+ stem cells were enriched and either 
      cultured for prenatal diagnosis or analyzed with fluorescence in situ 
      hybridization (FISH)/polymerase chain reaction (PCR) to determine peristance in 
      maternal blood. Fetal/maternal cells can be isolated and grown "in vitro" to 
      provide enough cells for a more accurate fetal sex or aneuploid prediction than 
      is provided by unenriched and uncultured CD34+ stem cells. The presence of fetal 
      cells in maternal blood samples from mothers who had given birth to male 
      offspring was found in 3 of 13 blood samples. PCR was positive for Y chromosome 
      in one woman who had never been pregnant. Analysis of cultured CD34+ stem cells 
      from mothers with Y PCR positivity did not detect any male cells in any samples. 
      Even if PCR positivity is due to persistence of fetal stem cells from previous 
      pregnancies, it does not seem to affect this new system of enrichment, culture, 
      and FISH analysis of CD34+ fetal stem cells.
FAU - Coata, G
AU  - Coata G
AD  - Centre of Perinatal and Reproductive Medicine, University of Perugia, Policlinico 
      Monteluce, Via Brunamonti, 06122 Perugia, Italy.
FAU - Tilesi, F
AU  - Tilesi F
FAU - Fizzotti, M
AU  - Fizzotti M
FAU - Lauro, V
AU  - Lauro V
FAU - Pennacchi, L
AU  - Pennacchi L
FAU - Tabilio, A
AU  - Tabilio A
FAU - Di Renzo, G C
AU  - Di Renzo GC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells
JT  - Stem cells (Dayton, Ohio)
JID - 9304532
RN  - 0 (Antigens, CD34)
SB  - IM
MH  - Adult
MH  - Antigens, CD34/*immunology
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Congenital Abnormalities/*blood/diagnosis/genetics
MH  - Female
MH  - Fetus
MH  - Hematopoietic Stem Cells/*cytology/immunology/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Pregnancy
MH  - *Prenatal Diagnosis
MH  - Trisomy
EDAT- 2001/11/20 10:00
MHDA- 2002/01/12 10:01
CRDT- 2001/11/20 10:00
PHST- 2001/11/20 10:00 [pubmed]
PHST- 2002/01/12 10:01 [medline]
PHST- 2001/11/20 10:00 [entrez]
AID - 10.1634/stemcells.19-6-534 [doi]
PST - ppublish
SO  - Stem Cells. 2001;19(6):534-42. doi: 10.1634/stemcells.19-6-534.