PMID- 11714757
OWN - NLM
STAT- MEDLINE
DCOM- 20011213
LR  - 20190508
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 194
IP  - 10
DP  - 2001 Nov 19
TI  - Intercellular transfer and supramolecular organization of human leukocyte antigen 
      C at inhibitory natural killer cell immune synapses.
PG  - 1507-17
AB  - After accumulation of target cell human leukocyte antigen (HLA)-C at inhibitory 
      natural killer (NK) cell immune synapses, some HLA-C transfers from target cells 
      to NK cell plasma membranes and cytoplasm. This unexpected intercellular transfer 
      of HLA-C is dependent on NK receptor recognition, since HLA-Cw6 or -Cw4 but not 
      -Cw3 transfer to an NK transfectant expressing killer Ig-like receptor (KIR)2DL1. 
      Strikingly, live-cell time-lapse laser scanning confocal microscopy shows 
      vesicles containing target cell green fluorescent protein-tagged HLA-C migrating 
      away from immune synapses into NK cells. Unlike clustering of HLA-C at the immune 
      synapse, intercellular transfer of HLA-C is dependent on NK cell ATP, but not 
      target cell ATP. However, the intercellular transfer of HLA-C is not dependent on 
      active polymerization of the actin cytoskeleton. In addition, different 
      arrangements of HLA-C are seen at inhibitory NK immune synapses, and these alter 
      as NK synapses mature, but in a fashion distinct from that seen upon T cell 
      activation.
FAU - Carlin, L M
AU  - Carlin LM
AD  - Department of Biological Sciences, Sir Alexander Fleming Building, Imperial 
      College of Science, Technology, and Medicine, South Kensington, London SW7 2AZ, 
      United Kingdom.
FAU - Eleme, K
AU  - Eleme K
FAU - McCann, F E
AU  - McCann FE
FAU - Davis, D M
AU  - Davis DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (HLA-C Antigens)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR2DL1)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
EIN - J Exp Med 2001 Dec 17;194(12):1883
MH  - Adenosine Triphosphate/metabolism
MH  - Biological Transport
MH  - Cell Line
MH  - Cytoskeleton/metabolism
MH  - HLA-C Antigens/chemistry/*metabolism
MH  - Humans
MH  - Killer Cells, Natural/*metabolism
MH  - Receptors, Immunologic/metabolism
MH  - Receptors, KIR2DL1
MH  - Synapses/*metabolism
PMC - PMC2193674
EDAT- 2001/11/21 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/05/19
CRDT- 2001/11/21 10:00
PHST- 2001/11/21 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/21 10:00 [entrez]
PHST- 2002/05/19 00:00 [pmc-release]
AID - 011232 [pii]
AID - 10.1084/jem.194.10.1507 [doi]
PST - ppublish
SO  - J Exp Med. 2001 Nov 19;194(10):1507-17. doi: 10.1084/jem.194.10.1507.