PMID- 11715462
OWN - NLM
STAT- MEDLINE
DCOM- 20020114
LR  - 20131121
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 79
IP  - 9
DP  - 1999 Sep
TI  - [The effects of L-arginine and nimodipine on the expression of monocyte 
      chemoattractant protein-1 in endothelial cells induced by lipopolysaccharide].
PG  - 699-701
AB  - OBJECTIVE: To examine the effects of L-arginine and nimodipine on the expression 
      of monocyte chemoattractant protein-1 (MCP-1) mRNA and protein induced by 
      lipopolysaccharide (LPS) in endothelial cells (ECs). METHODS: After 8-hour 
      exposure to LPS, LPS + L-arginine and LPS + nimodipine respectively, the total 
      RNA in ECs was extracted by the guanidinium isothiocyanate method. The MCP-1 mRNA 
      expression in ECs was examined by Dot blot analysis using a gamma-32P-end-labeled 
      35 mer oligonucleotide probe of MCP-1. MCP-1 protein in the EC-conditioned media 
      (EC-CM) was determined by sandwich ELISA for each group. The expression of MCP-1 
      protein in ECs was examined immunocytochemically. RESULTS: Dot blotting showed 
      that after exposure to LPS, the integral absorbance (A) value of the dots of 
      MCP-1 mRNA on the nitrocellulose membrane were 3.14, which was 2.5-fold as much 
      as that in the control group (A = 1.24). The A values of the LPS + L-arginine 
      group and the LPS + nimodipine group were 1.43 and 1.65, respectively. ELISA 
      showed that the MCP-1 protein content in the EC-CM of the LPS group was 6.29 +/- 
      0.53 ng/ml, which was markedly higher than that of the control group (2.32 +/- 
      0.16) ng/ml (P < 0.01). The MCP-1 protein content in the EC-CM of the LPS + 
      L-arginine group and the LPS + nimodipine group was significantly lower than that 
      of the LPS group (P < 0.01). Furthermore, the ECs in LPS group showed a strong 
      immunoreactivity for the polyclonal MCP-1 antibody, whereas the ECs in control, 
      L-arginine and nimodipine groups revealed a weak postive immunostaining. 
      CONCLUSION: L-arginine and nimodipine can markedly inhibit the expression of 
      MCP-1 in ECs induced by LPS.
FAU - Yu, G
AU  - Yu G
AD  - Department of Pathology, Tongji Medical University, Wuhan 430030.
FAU - Deng, Z
AU  - Deng Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 57WA9QZ5WH (Nimodipine)
RN  - 94ZLA3W45F (Arginine)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/cytology
MH  - Arginine/*pharmacology
MH  - Calcium Channel Blockers/*pharmacology
MH  - Cattle
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Nimodipine/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
EDAT- 2001/11/22 10:00
MHDA- 2002/01/15 10:01
CRDT- 2001/11/22 10:00
PHST- 2001/11/22 10:00 [pubmed]
PHST- 2002/01/15 10:01 [medline]
PHST- 2001/11/22 10:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 1999 Sep;79(9):699-701.