PMID- 11716482
OWN - NLM
STAT- MEDLINE
DCOM- 20020124
LR  - 20131121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 289
IP  - 2
DP  - 2001 Nov 30
TI  - Brucella lipopolysaccharides induce cyclooxygenase-2 expression in monocytic 
      cells.
PG  - 372-5
AB  - Human brucellosis is characterized by the presence of both acute inflammatory 
      episodes and chronic inflammation with granuloma formation. On this basis, the 
      proinflammatory effects of smooth lipopolysaccharide of Brucella (S-LPS) were 
      addressed and compared to those of LPS from Escherichia coli. For this purpose, 
      the induction of cyclooxygenase-2 (COX-2), the production of the chemokine 
      monocyte chemoattractant protein-1 (MCP-1), and the activation of the nuclear 
      factor kappa B (NF-kappa B) were studied. S-LPS was found to induce both COX-2 
      expression and MCP-1 production; however, the potency of E. coli LPS exceeded 
      that of Brucella S-LPS by some orders of magnitude. However, at concentrations 
      above 1 microg/ml, all of the LPS produced comparable effects, including their 
      ability to activate the NF-kappa B system. These observations help explain the 
      inflammatory events associated with Brucella infection and the ability of 
      Brucella to produce monocyte recruitment and granuloma formation.
FAU - Lopez-Urrutia, L
AU  - Lopez-Urrutia L
AD  - Departamento de Microbiologia, Facultad de Medicina, Instituo de Biologia y 
      Genetica Molecular, CSIC, Valladolid, Spain.
FAU - Alonso, A
AU  - Alonso A
FAU - Bayon, Y
AU  - Bayon Y
FAU - Nieto, M L
AU  - Nieto ML
FAU - Orduna, A
AU  - Orduna A
FAU - Sanchez Crespo, M
AU  - Sanchez Crespo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-kappa B)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
SB  - IM
MH  - Arachidonic Acid/metabolism
MH  - Blotting, Western
MH  - Brucella/metabolism
MH  - Brucella abortus/*metabolism
MH  - Brucella melitensis/*metabolism
MH  - Brucellosis/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Cyclooxygenase 2
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation
MH  - Escherichia coli/metabolism
MH  - Granuloma/metabolism
MH  - Humans
MH  - I-kappa B Proteins/metabolism
MH  - Isoenzymes/*biosynthesis
MH  - Leukocytes/metabolism
MH  - Lipopolysaccharides/*metabolism
MH  - Membrane Proteins
MH  - Monocytes/*enzymology/metabolism
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/metabolism
MH  - Prostaglandin-Endoperoxide Synthases/*biosynthesis
MH  - Time Factors
EDAT- 2001/11/22 10:00
MHDA- 2002/01/25 10:01
CRDT- 2001/11/22 10:00
PHST- 2001/11/22 10:00 [pubmed]
PHST- 2002/01/25 10:01 [medline]
PHST- 2001/11/22 10:00 [entrez]
AID - S0006-291X(01)95995-9 [pii]
AID - 10.1006/bbrc.2001.5995 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2001 Nov 30;289(2):372-5. doi: 
      10.1006/bbrc.2001.5995.