PMID- 11716693
OWN - NLM
STAT- MEDLINE
DCOM- 20020226
LR  - 20220309
IS  - 1043-1802 (Print)
IS  - 1043-1802 (Linking)
VI  - 12
IP  - 6
DP  - 2001 Nov-Dec
TI  - Stearylated arginine-rich peptides: a new class of transfection systems.
PG  - 1005-11
AB  - Membrane-permeable arginine-rich peptides, such as HIV-1 Tat-(48-60), HIV-1 
      Rev-(34-50), and flock house virus (FHV) coat-(35-49), have been shown to possess 
      the ability to transfect COS-7 cells with luciferase-coding plasmid as 
      efficiently as polyarginine (MW 5000-15 000) and polylysine (MW 9800). Not only 
      these virus-derived cationic peptides but also oligoarginines of 4-16 residues 
      were found to be able to transfect cells. In the case of the Tat, FHV, and 
      octaarginine peptides, N-terminal stearylation of the peptides increases the 
      transfection efficiency by approximately 100 times to reach the same order of 
      magnitude as that of LipofectAMINE, one of the most efficient commercially 
      available transfection agents. Also, a certain correlation was observed between 
      the transfection efficiency of stearyl-(Arg)n peptides (stearyl-Rn: n = 4, 8, 12, 
      16) and the membrane permeability of the corresponding (Arg)n peptides (Rn).
FAU - Futaki, S
AU  - Futaki S
AD  - Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan. 
      futaki@scl.kyoto-u.ac.jp
FAU - Ohashi, W
AU  - Ohashi W
FAU - Suzuki, T
AU  - Suzuki T
FAU - Niwa, M
AU  - Niwa M
FAU - Tanaka, S
AU  - Tanaka S
FAU - Ueda, K
AU  - Ueda K
FAU - Harashima, H
AU  - Harashima H
FAU - Sugiura, Y
AU  - Sugiura Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Bioconjug Chem
JT  - Bioconjugate chemistry
JID - 9010319
RN  - 0 (Gene Products, rev)
RN  - 0 (Gene Products, tat)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Fragments)
RN  - 0 (Peptides)
RN  - 0 (Stearic Acids)
RN  - 0 (Viral Proteins)
RN  - 0 (tat Gene Products, Human Immunodeficiency Virus)
RN  - 0 (tat peptide (32-72), Human immunodeficiency virus 1)
RN  - 25212-18-4 (polyarginine)
RN  - 4ELV7Z65AP (stearic acid)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 94ZLA3W45F (Arginine)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Arginine/*chemistry
MH  - Biological Transport
MH  - COS Cells
MH  - Gene Products, rev/chemistry
MH  - Gene Products, tat/chemistry
MH  - Humans
MH  - Luciferases/genetics/metabolism
MH  - Molecular Sequence Data
MH  - Nodaviridae/chemistry
MH  - Oligopeptides/chemistry
MH  - Peptide Fragments/chemistry
MH  - Peptides/*chemistry
MH  - Plasmids
MH  - Stearic Acids/*chemistry
MH  - Transfection/*methods
MH  - Viral Proteins/chemistry
MH  - tat Gene Products, Human Immunodeficiency Virus
EDAT- 2001/11/22 10:00
MHDA- 2002/02/28 10:01
CRDT- 2001/11/22 10:00
PHST- 2001/11/22 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2001/11/22 10:00 [entrez]
AID - bc015508l [pii]
AID - 10.1021/bc015508l [doi]
PST - ppublish
SO  - Bioconjug Chem. 2001 Nov-Dec;12(6):1005-11. doi: 10.1021/bc015508l.