PMID- 11717365
OWN - NLM
STAT- MEDLINE
DCOM- 20020111
LR  - 20220309
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 21
IP  - 23
DP  - 2001 Dec 1
TI  - Regional differences in neurotrophin availability regulate selective expression 
      of VGF in the developing limbic cortex.
PG  - 9315-24
AB  - Gene and protein expression patterns in the cerebral cortex are complex and often 
      change spatially and temporally through development. The signals that regulate 
      these patterns are primarily unknown. In the present study, we focus on the 
      regulation of VGF expression, which is limited to limbic cortical areas early in 
      development but later expands into sensory and motor areas. We isolated neurons 
      from embryonic day 17 rat cortex and demonstrate that the profile of VGF 
      expression in perirhinal (expressing) and occipital (nonexpressing) populations 
      in vitro is similar to that in the perinatal cortex in vivo. The addition of 
      neutralizing neurotrophin antibodies indicates that endogenous brain-derived 
      neurotrophic factor (BDNF) is necessary for the normal complement of 
      VGF-expressing neurons in the perirhinal cortex, although endogenous 
      neurotrophin-3 (NT-3) regulates the expression of VGF in a subpopulation of 
      cells. ELISA analysis demonstrates that there is significantly more BDNF present 
      in the perirhinal cortex compared with the occipital cortex in the perinatal 
      period. However, the total amount of NT-3 is similar between the two regions and, 
      moreover, there is considerably more NT-3 than BDNF in both areas, a finding 
      seemingly in conflict with regional VGF expression. Quantification of the 
      extracellular levels of neurotrophins in perirhinal and occipital cultures using 
      ELISA in situ analysis indicates that perirhinal neurons release significantly 
      more BDNF than the occipital population. Furthermore, the amount of NT-3 released 
      by the perirhinal neurons is significantly less than the amount of BDNF. Local 
      injection of BDNF in vivo into a normally negative VGF region results in robust 
      ectopic expression of VGF. These data suggest that the local availability of 
      specific neurotrophins for receptor occupation, rather than the total amount of 
      neurotrophin, is a critical parameter in determining the selective expression of 
      VGF in the developing limbic cortex.
FAU - Eagleson, K L
AU  - Eagleson KL
AD  - Department of Neurobiology, University of Pittsburgh School of Medicine, 
      Pittsburgh, Pennsylvania 15261, USA. keagle+@pitt.edu
FAU - Fairfull, L D
AU  - Fairfull LD
FAU - Salton, S R
AU  - Salton SR
FAU - Levitt, P
AU  - Levitt P
LA  - eng
GR  - AG 10676/AG/NIA NIH HHS/United States
GR  - MH 45507/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuropeptides)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vgf protein, rat)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Brain-Derived Neurotrophic Factor/administration & dosage/metabolism
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/embryology/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Regulation, Developmental/drug effects/physiology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Limbic System/cytology/embryology/*metabolism
MH  - Microinjections
MH  - Nerve Growth Factors/antagonists & inhibitors/*metabolism/pharmacology
MH  - Neurons/cytology/drug effects/metabolism
MH  - Neuropeptides
MH  - Neurotrophin 3/metabolism
MH  - Occipital Lobe/cytology/embryology/metabolism
MH  - Parahippocampal Gyrus/cytology/embryology/metabolism
MH  - Proteins/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Tissue Distribution
PMC - PMC6763919
EDAT- 2001/11/22 10:00
MHDA- 2002/01/12 10:01
PMCR- 2002/06/01
CRDT- 2001/11/22 10:00
PHST- 2001/11/22 10:00 [pubmed]
PHST- 2002/01/12 10:01 [medline]
PHST- 2001/11/22 10:00 [entrez]
PHST- 2002/06/01 00:00 [pmc-release]
AID - 21/23/9315 [pii]
AID - 5850 [pii]
AID - 10.1523/JNEUROSCI.21-23-09315.2001 [doi]
PST - ppublish
SO  - J Neurosci. 2001 Dec 1;21(23):9315-24. doi: 10.1523/JNEUROSCI.21-23-09315.2001.