PMID- 11721447
OWN - NLM
STAT- MEDLINE
DCOM- 20020124
LR  - 20101118
IS  - 0366-6999 (Print)
IS  - 0366-6999 (Linking)
VI  - 112
IP  - 12
DP  - 1999 Dec
TI  - Up-regulation of phosphatidylinositol 3-kinase in psoriatic lesions.
PG  - 1097-100
AB  - OBJECTIVE: To explore the relationship between phosphatidylinositol 3-kinase (PI 
      3-kinase) and psoriasis. METHODS: We constructed eukaryotic expression vector 
      PcDNA 3-PI 3-kinase, by which digoxin-labeled RNA probe of PI 3-kinase was 
      generated. After that, we detected the expressions of PI 3-kinase in the skins of 
      twelve patients with plaque psoriasis and five normal subjects by dot blot, in 
      situ hybridization and immunohistochemistry. RESULTS: The expression of PI 
      3-kinase mRNA and protein in psoriatic lesions increased obviously compared with 
      that in normal skin. The expression of the PI 3-kinase gene conformed to the 
      expression of its protein in psoriatic and normal human skins. CONCLUSIONS: 
      Over-expression of PI 3-kinase may be related to the hyperproliferation of 
      psoriatic keratinocytes. Transfection of the anti-sense gene of PI 3-kinase into 
      psoriatic keratinocytes might be a best way to elucidate the issue.
FAU - Zhang, X
AU  - Zhang X
AD  - Department of Biology, Beijing Normal University, Beijing 100875, China.
FAU - Liu, H
AU  - Liu H
FAU - Ma, S
AU  - Ma S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Chin Med J (Engl)
JT  - Chinese medical journal
JID - 7513795
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.1.- (Phosphatidylinositol 3-Kinases)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Infant
MH  - Keratinocytes/pathology
MH  - Male
MH  - Middle Aged
MH  - Phosphatidylinositol 3-Kinases/*biosynthesis/genetics
MH  - Psoriasis/*enzymology/genetics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Skin/*enzymology/pathology
MH  - Up-Regulation
EDAT- 2001/11/28 10:00
MHDA- 2002/01/25 10:01
CRDT- 2001/11/28 10:00
PHST- 2001/11/28 10:00 [pubmed]
PHST- 2002/01/25 10:01 [medline]
PHST- 2001/11/28 10:00 [entrez]
PST - ppublish
SO  - Chin Med J (Engl). 1999 Dec;112(12):1097-100.