PMID- 11722179
OWN - NLM
STAT- MEDLINE
DCOM- 20020430
LR  - 20051117
IS  - 1046-5928 (Print)
IS  - 1046-5928 (Linking)
VI  - 23
IP  - 3
DP  - 2001 Dec
TI  - Purification of human recombinant GATA-1 from bacteria: implication for 
      protein-protein interaction studies.
PG  - 426-31
AB  - GATA-1 is a key regulator of terminal erythroid differentiation in mammals and 
      birds. The structural and biochemical studies of human GATA-1 (hGATA-1) are 
      limited by the difficulty of its purification in a sufficient amount. Here we 
      describe the procedure for obtaining pure bacterial recombinant hGATA-1 in an 
      active functional state. We demonstrate that this protein may be successfully 
      used for preparing an affinity column, producing GATA-1-specific rabbit 
      polyclonal antibodies, and studying DNA-protein and protein-protein interactions.
CI  - Copyright 2001 Elsevier Science.
FAU - Doubeikovskaia, Z
AU  - Doubeikovskaia Z
AD  - Institute Biochemistry and Physiology of Microorganisms, Russian Academy of 
      Sciences, Prospekt Nauki, 5, Pushchino Moscow Region, 142290, Russia. 
      douba@rambler.ru
FAU - Aries, A
AU  - Aries A
FAU - Jeannesson, P
AU  - Jeannesson P
FAU - Morle, F
AU  - Morle F
FAU - Doubeikovski, A
AU  - Doubeikovski A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Protein Expr Purif
JT  - Protein expression and purification
JID - 9101496
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (GATA1 protein, human)
RN  - 0 (Gata1 protein, mouse)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Binding Sites
MH  - Blotting, Western
MH  - DNA-Binding Proteins/genetics/*isolation & purification/*metabolism
MH  - Erythroid-Specific DNA-Binding Factors
MH  - Escherichia coli/*genetics
MH  - GATA1 Transcription Factor
MH  - Gene Expression
MH  - Humans
MH  - Inclusion Bodies/metabolism
MH  - Leukemia, Erythroblastic, Acute/metabolism
MH  - Mice
MH  - Protein Binding
MH  - Rabbits
MH  - Recombinant Fusion Proteins/chemistry/isolation & purification/metabolism
MH  - Solubility
MH  - Transcription Factors/genetics/*isolation & purification/*metabolism
MH  - Transfection
MH  - Transformation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2001/11/28 10:00
MHDA- 2002/05/01 10:01
CRDT- 2001/11/28 10:00
PHST- 2001/11/28 10:00 [pubmed]
PHST- 2002/05/01 10:01 [medline]
PHST- 2001/11/28 10:00 [entrez]
AID - S1046-5928(01)91533-5 [pii]
AID - 10.1006/prep.2001.1533 [doi]
PST - ppublish
SO  - Protein Expr Purif. 2001 Dec;23(3):426-31. doi: 10.1006/prep.2001.1533.