PMID- 11722640
OWN - NLM
STAT- MEDLINE
DCOM- 20011214
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 104
IP  - 3
DP  - 2001 Nov
TI  - Contribution of conformational stability of hen lysozyme to induction of type 2 
      T-helper immune responses.
PG  - 259-68
AB  - It is important to identify characteristics that confer on proteins the potential 
      to induce allergenic sensitization and allergenic disease. Protein allergens 
      carry T-cell epitopes that are capable of inducing a type 2 T helper (Th2) cell 
      response. There is limited information regarding factors that govern the 
      allergenicity of proteins. We previously reported that a decrease in the 
      conformational stability of hen-egg lysozyme (HEL) enhanced its capacity to 
      activate HEL-specific T cells owing to the increased susceptibility to 
      intracellular antigen processing. To determine whether the conformational 
      stability of HEL makes for a critical contribution to allergenic sensitization in 
      vivo, we immunized BALB/c mice with HEL derivatives of different conformational 
      stability, but which retained a similar three-dimensional structure. The 
      magnitude of in vivo T-cell responses, evaluated by ex vivo proliferative 
      responses of lymph node T cells from mice primed with various HEL derivatives, 
      was inversely correlated with conformational stability, as was interferon-gamma 
      (IFN-gamma) and interleukin-4 (IL-4) production by splenic T cells in response to 
      HEL. Immunization of the least stable derivative led to a potent IL-4 response 
      and to immunoglobulin E (IgE) antibody production. We propose that the intrinsic 
      allergenicity of proteins can be determined by the degree of conformational 
      stability.
FAU - So, T
AU  - So T
AD  - Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
FAU - Ito, H
AU  - Ito H
FAU - Hirata, M
AU  - Hirata M
FAU - Ueda, T
AU  - Ueda T
FAU - Imoto, T
AU  - Imoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.2.1.- (hen egg lysozyme)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/immunology
MH  - Antigens/immunology
MH  - Epitopes, T-Lymphocyte/immunology
MH  - Female
MH  - Immunoglobulin E/biosynthesis
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Muramidase/chemistry/*immunology
MH  - Protein Conformation
MH  - Structure-Activity Relationship
MH  - Th1 Cells/immunology
MH  - Th2 Cells/*immunology
PMC - PMC1783307
EDAT- 2001/11/28 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/11/01
CRDT- 2001/11/28 10:00
PHST- 2001/11/28 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/28 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 1314 [pii]
AID - 10.1046/j.1365-2567.2001.01314.x [doi]
PST - ppublish
SO  - Immunology. 2001 Nov;104(3):259-68. doi: 10.1046/j.1365-2567.2001.01314.x.