PMID- 11722648
OWN - NLM
STAT- MEDLINE
DCOM- 20011214
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 104
IP  - 3
DP  - 2001 Nov
TI  - Rat mast cell protease-I enhances immunoglobulin E production by mouse B cells 
      stimulated with interleukin-4.
PG  - 333-40
AB  - Mast cell chymase plays important roles in inflammation and tissue remodeling. 
      Here we show that mast cell chymase also functions as an enhancer of 
      immunoglobulin production. In the culture of murine spleen cells stimulated with 
      lipopolysaccharide and interleukin-4, purified rat chymase (rat mast cell 
      protease-I; RMCP-I), at physiological concentrations, enhanced immunoglobulin E 
      (IgE) and IgG1 syntheses but not IgG3 synthesis. The enhancement was also evident 
      when spleen cells depleted of T cells and macrophages were employed as responding 
      cells. Enzymatic activity of RMCP-I was required to enhance IgE and IgG1, because 
      two inhibitors for chymotryptic enzymes, chymostatin and Y-40613, a novel chymase 
      inhibitor, suppressed the enhanced immunoglobulin production, and 
      phenylmethylsulphonyl fluoride, an irreversible inhibitor for serine proteases, 
      totally abolished the enhancing effect. Furthermore, a specific inhibitor for 
      Zn2+-dependent metalloproteases, GI 129471, could also completely inhibit the 
      production of IgE and IgG1 that was enhanced by RMCP-I, suggesting that a 
      metalloprotease also played an essential role in the immunoglobulin production. 
      Our results together with others show that proteases from mast cell granules have 
      important function not only in the efferent phase but also in the afferent phase 
      of immune responses.
FAU - Yoshikawa, T
AU  - Yoshikawa T
AD  - Pharmaceutical Research Division, Mitsubishi Pharma Corporation, Osaka, Japan.
FAU - Imada, T
AU  - Imada T
FAU - Nakakubo, H
AU  - Nakakubo H
FAU - Nakamura, N
AU  - Nakamura N
FAU - Naito, K
AU  - Naito K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Immunoglobulin G)
RN  - 0 (Matrix Metalloproteinase Inhibitors)
RN  - 0 (Serine Proteinase Inhibitors)
RN  - 130370-59-1 (GI 129471)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 47E5O17Y3R (Phenylalanine)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.39 (Chymases)
RN  - EC 3.4.24.- (Matrix Metalloproteinases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Cattle
MH  - Cell Culture Techniques
MH  - Chymases
MH  - Dose-Response Relationship, Immunologic
MH  - Female
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/biosynthesis
MH  - Interleukin-4/*immunology
MH  - Lymphocyte Activation/immunology
MH  - Mast Cells/enzymology/immunology
MH  - Matrix Metalloproteinase 1
MH  - Matrix Metalloproteinase Inhibitors
MH  - Matrix Metalloproteinases/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phenylalanine/*analogs & derivatives/pharmacology
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Serine Endopeptidases/*immunology
MH  - Serine Proteinase Inhibitors/pharmacology
MH  - Spleen/immunology
PMC - PMC1783305
EDAT- 2001/11/28 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/11/01
CRDT- 2001/11/28 10:00
PHST- 2001/11/28 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/28 10:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 1320 [pii]
AID - 10.1046/j.1365-2567.2001.01320.x [doi]
PST - ppublish
SO  - Immunology. 2001 Nov;104(3):333-40. doi: 10.1046/j.1365-2567.2001.01320.x.