PMID- 11722890
OWN - NLM
STAT- MEDLINE
DCOM- 20020304
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 67
IP  - 12
DP  - 2001 Dec
TI  - Consumption of tropospheric levels of methyl bromide by C(1) compound-utilizing 
      bacteria and comparison to saturation kinetics.
PG  - 5437-43
AB  - Pure cultures of methylotrophs and methanotrophs are known to oxidize methyl 
      bromide (MeBr); however, their ability to oxidize tropospheric concentrations 
      (parts per trillion by volume [pptv]) has not been tested. Methylotrophs and 
      methanotrophs were able to consume MeBr provided at levels that mimicked the 
      tropospheric mixing ratio of MeBr (12 pptv) at equilibrium with surface waters ( 
      approximately 2 pM). Kinetic investigations using picomolar concentrations of 
      MeBr in a continuously stirred tank reactor (CSTR) were performed using strain 
      IMB-1 and Leisingeria methylohalidivorans strain MB2(T) - terrestrial and marine 
      methylotrophs capable of halorespiration. First-order uptake of MeBr with no 
      indication of threshold was observed for both strains. Strain MB2(T) displayed 
      saturation kinetics in batch experiments using micromolar MeBr concentrations, 
      with an apparent K(s) of 2.4 microM MeBr and a V(max) of 1.6 nmol h(-1) (10(6) 
      cells)(-1). Apparent first-order degradation rate constants measured with the 
      CSTR were consistent with kinetic parameters determined in batch experiments, 
      which used 35- to 1 x 10(7)-fold-higher MeBr concentrations. Ruegeria algicola (a 
      phylogenetic relative of strain MB2(T)), the common heterotrophs Escherichia coli 
      and Bacillus pumilus, and a toluene oxidizer, Pseudomonas mendocina KR1, were 
      also tested. These bacteria showed no significant consumption of 12 pptv MeBr; 
      thus, the ability to consume ambient mixing ratios of MeBr was limited to C(1) 
      compound-oxidizing bacteria in this study. Aerobic C(1) bacteria may provide 
      model organisms for the biological oxidation of tropospheric MeBr in soils and 
      waters.
FAU - Goodwin, K D
AU  - Goodwin KD
AD  - Cooperative Institute for Marine and Atmospheric Studies, Rosenstiel School of 
      Marine and Atmospheric Sciences, University of Miami, Miami, Florida 33149, USA. 
      kelly.goodwin@noaa.gov
FAU - Varner, R K
AU  - Varner RK
FAU - Crill, P M
AU  - Crill PM
FAU - Oremland, R S
AU  - Oremland RS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Culture Media)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 9V42E1Z7B6 (methyl bromide)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Atmosphere/*chemistry
MH  - Bacteria/growth & development/*metabolism
MH  - Culture Media
MH  - Hydrocarbons, Brominated/*metabolism
MH  - Methane/metabolism
MH  - Oxidation-Reduction
MH  - Seawater/microbiology
MH  - *Soil Microbiology
PMC - PMC93327
EDAT- 2001/11/28 10:00
MHDA- 2002/03/05 10:01
PMCR- 2001/12/01
CRDT- 2001/11/28 10:00
PHST- 2001/11/28 10:00 [pubmed]
PHST- 2002/03/05 10:01 [medline]
PHST- 2001/11/28 10:00 [entrez]
PHST- 2001/12/01 00:00 [pmc-release]
AID - 1127 [pii]
AID - 10.1128/AEM.67.12.5437-5443.2001 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2001 Dec;67(12):5437-43. doi: 
      10.1128/AEM.67.12.5437-5443.2001.