PMID- 11723167
OWN - NLM
STAT- MEDLINE
DCOM- 20011220
LR  - 20190630
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 79
IP  - 4
DP  - 2001 Nov
TI  - Brain-derived neurotrophic factor (BDNF) mediates bone morphogenetic protein-2 
      (BMP-2) effects on cultured striatal neurones.
PG  - 747-55
AB  - Bone morphogenetic proteins are members of the transforming growth factor-beta 
      superfamily that have multiple functions in the developing nervous system. One of 
      them, bone morphogenetic protein-2 (BMP-2), promotes the differentiation of 
      cultured striatal neurones, enhancing dendrite growth and calbindin-positive 
      phenotype. Bone morphogenetic proteins have been implicated in cooperative 
      interactions with other neurotrophic factors. Here we examined whether the 
      effects of BMP-2 on cultured striatal neurones are mediated or enhanced by other 
      neurotrophic factors. BMP-2 had a cooperative effect with low doses of 
      brain-derived neurotrophic factor or neurotrophin-3 (but not with other 
      neurotrophic factors such as glial cell line-derived neurotrophic factor, 
      neurturin or transforming growth factor-beta 2) on the number of 
      calbindin-positive striatal neurones. Moreover, BMP-2 induced phosphorylated Trk 
      immunoreactivity in cultured striatal neurones, suggesting that neurotrophins are 
      involved in BMP-2 neurotrophic effects. The addition of TrkB-IgG or antibodies 
      against brain-derived neurotrophic factor abolished the effects of BMP-2 on the 
      number and degree of differentiation of calbindin-positive striatal neurones. 
      Indeed, BMP-2 treatment increased brain-derived neurotrophic factor protein 
      levels in treated cultures media and BDNF immunocytochemistry revealed that this 
      neurotrophin was produced by neuronal cells. Taken together, these results 
      indicate that brain-derived neurotrophic factor mediates the effects of BMP-2 on 
      striatal neurones.
FAU - Gratacos, E
AU  - Gratacos E
AD  - Departament de Biologia Cel.lular i Anatomia Patologica, Facultat de Medicina, 
      Institut d'Investigacions Biomediques August Pi i Sunyer, Universitat de 
      Barcelona, Barcelona, Spain.
FAU - Checa, N
AU  - Checa N
FAU - Perez-Navarro, E
AU  - Perez-Navarro E
FAU - Alberch, J
AU  - Alberch J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Antibodies)
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calbindins)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta2)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Antibodies/pharmacology
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/antagonists & 
      inhibitors/metabolism/*pharmacology
MH  - Calbindins
MH  - Cell Count
MH  - Corpus Striatum/cytology/drug effects/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Drug Synergism
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - *Nerve Growth Factors
MH  - Nerve Tissue Proteins/pharmacology
MH  - Neurons/cytology/drug effects/*metabolism
MH  - Neurotrophin 3/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkB/metabolism
MH  - S100 Calcium Binding Protein G/metabolism
MH  - Transforming Growth Factor beta/pharmacology
MH  - Transforming Growth Factor beta2
MH  - gamma-Aminobutyric Acid/pharmacokinetics
EDAT- 2001/11/28 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/11/28 10:00
PHST- 2001/11/28 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/11/28 10:00 [entrez]
AID - 10.1046/j.1471-4159.2001.00570.x [doi]
PST - ppublish
SO  - J Neurochem. 2001 Nov;79(4):747-55. doi: 10.1046/j.1471-4159.2001.00570.x.