PMID- 11724751
OWN - NLM
STAT- MEDLINE
DCOM- 20020114
LR  - 20181113
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 134
IP  - 7
DP  - 2001 Dec
TI  - In vitro neuronal and vascular responses to 5-HT in rats chronically exposed to 
      MDMA.
PG  - 1455-60
AB  - 1. This study examined the effects of chronic exposure of rats to 
      3,4-methylenedioxymethamphetamine (MDMA) on [(3)H]5-hydroxytryptamine 
      ([(3)H]5-HT) re-uptake into purified rat brain synaptosomes, 5-HT-induced 
      isometric contraction of aortic rings and [(3)H]5-HT re-uptake into rat aorta. 2. 
      Rats were administered MDMA (20 mg kg(-1) i.p.) twice daily over 4 days. One, 7, 
      14 or 21 days post treatment, whole brain synaptosomes and descending thoracic 
      aortic rings were prepared for investigation. 3. Chronic MDMA treatment 
      significantly reduced the maximum rate (V(max)) of specific high-affinity 
      [(3)H]5-HT re-uptake 1 day after treatment and for up to 21 days post-final 
      administration of MDMA. Direct application of MDMA (100 microM) abolished 
      synaptosomal re-uptake of [(3)H]5-HT in vitro. 4. Chronic MDMA administration 
      significantly reduced the maximum contraction (E(max)) to 5-HT at 1 and 7 days 
      after treatment, but not at 14 or 21 days. 5. Chronic MDMA administration had no 
      effect on sodium-dependent [(3)H]5-HT re-uptake into aorta 1 day after treatment, 
      nor did 100 microM MDMA have any direct effect on [(3)H]5-HT uptake into aortic 
      rings in vitro. 6. These results show, for the first time, an altered 
      responsiveness of vascular tissue to MDMA after chronic administration. In 
      addition, they demonstrate a difference in the sensitivity of central and 
      peripheral 5-HT uptake systems to chronic MDMA exposure, and suggest that the 
      action of MDMA in the cardiovascular system does not arise from a direct effect 
      of MDMA on peripheral 5-HT transport.
FAU - Cannon, D M
AU  - Cannon DM
AD  - Department of Pharmacology, Conway Institute of Biomolecular and Biomedical 
      Research, University College Dublin, Belfield, Dublin 4, Ireland.
FAU - Keenan, A K
AU  - Keenan AK
FAU - Guiry, P J
AU  - Guiry PJ
FAU - Buon, C
AU  - Buon C
FAU - Baird, A W
AU  - Baird AW
FAU - McBean, G J
AU  - McBean GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 10028-17-8 (Tritium)
RN  - 333DO1RDJY (Serotonin)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Animals
MH  - Aorta, Thoracic/*drug effects/metabolism/physiology
MH  - Biological Transport/drug effects
MH  - Dose-Response Relationship, Drug
MH  - In Vitro Techniques
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Serotonin/*pharmacokinetics/pharmacology
MH  - Synaptosomes/*drug effects/metabolism
MH  - Tritium
MH  - Vasoconstriction/drug effects
PMC - PMC1573088
EDAT- 2001/11/29 10:00
MHDA- 2002/01/15 10:01
PMCR- 2002/12/01
CRDT- 2001/11/29 10:00
PHST- 2001/11/29 10:00 [pubmed]
PHST- 2002/01/15 10:01 [medline]
PHST- 2001/11/29 10:00 [entrez]
PHST- 2002/12/01 00:00 [pmc-release]
AID - 0704402 [pii]
AID - 10.1038/sj.bjp.0704402 [doi]
PST - ppublish
SO  - Br J Pharmacol. 2001 Dec;134(7):1455-60. doi: 10.1038/sj.bjp.0704402.