PMID- 11724869
OWN - NLM
STAT- MEDLINE
DCOM- 20020214
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 39
IP  - 12
DP  - 2001 Dec
TI  - Beyond serotypes and virulence-associated factors: detection of genetic diversity 
      among O153:H45 CFA/I heat-stable enterotoxigenic Escherichia coli strains.
PG  - 4500-5
AB  - Characterization of enterotoxigenic Escherichia coli (ETEC) has been based almost 
      exclusively on the detection of phenotypic traits such as serotypes and 
      virulence-associated factors: heat-labile (LT) and heat-stable (ST) toxins and 
      colonization factors (CFs). In the present work we show that the analysis of band 
      patterns generated by randomly amplified polymorphic DNA (RAPD) analysis and 
      pulsed-field gel electrophoresis (PFGE) of digested chromosomal DNA can be used 
      to detect genetic diversity among ETEC strains expressing identical phenotypic 
      traits. The study included 29 ETEC isolates from Latin America and Spain 
      expressing the phenotype O153:H45 CFA/I ST plus 1 rough derivative, 2 nonmotile 
      derivatives, and 1 O78:H12 CFA/I ST isolate, and a representative of a 
      genetically distinct ETEC group. The results showed that the O153:H45 CFA/I ST 
      ETEC isolates belong to a single clonal cluster whose isolates share on average, 
      84% of the RAPD bands and 77% of the PFGE restriction fragments, while the 
      O78:H12 isolate shared only 44 and 4% of the RAPD bands and PFGE fragments, 
      respectively, with the isolates of the O153:H45 group. More relevantly, RAPD and 
      PFGE fingerprints disclosed the presence of different clonal lineages among the 
      isolates of the O153:H45 cluster. Some of the genetic variants were isolated from 
      defined geographic areas, while places like Sao Paulo City in Brazil and the 
      middle-eastern part of Argentina were populated by several genetic variants of 
      related, but not identical, ETEC strains. These results show that molecular 
      biology-based typing methods can disclose strain diversity, which is usually 
      missed in studies restricted to phenotypic typing of ETEC.
FAU - Pacheco, A B
AU  - Pacheco AB
AD  - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de 
      Janeiro, Rio de Janeiro, RJ, 21949-900, Brazil. biafp@biof.ufrj.br
FAU - Ferreira, L C
AU  - Ferreira LC
FAU - Pichel, M G
AU  - Pichel MG
FAU - Almeida, D F
AU  - Almeida DF
FAU - Binsztein, N
AU  - Binsztein N
FAU - Viboud, G I
AU  - Viboud GI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Proteins)
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (colonization factor antigens)
RN  - 0 (heat stable toxin (E coli))
RN  - 147680-16-8 (Fimbriae Proteins)
SB  - IM
MH  - Bacterial Proteins/metabolism
MH  - Bacterial Toxins/metabolism
MH  - *Bacterial Typing Techniques
MH  - DNA, Bacterial/analysis
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Enterotoxins/metabolism
MH  - Escherichia coli/*classification/*genetics/pathogenicity
MH  - Escherichia coli Infections/*microbiology
MH  - Escherichia coli Proteins
MH  - *Fimbriae Proteins
MH  - *Genetic Variation
MH  - Humans
MH  - Phenotype
MH  - *Random Amplified Polymorphic DNA Technique
MH  - Serotyping
MH  - Virulence
PMC - PMC88573
EDAT- 2001/11/29 10:00
MHDA- 2002/02/15 10:01
PMCR- 2001/12/01
CRDT- 2001/11/29 10:00
PHST- 2001/11/29 10:00 [pubmed]
PHST- 2002/02/15 10:01 [medline]
PHST- 2001/11/29 10:00 [entrez]
PHST- 2001/12/01 00:00 [pmc-release]
AID - 0624 [pii]
AID - 10.1128/JCM.39.12.4500-4505.2001 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2001 Dec;39(12):4500-5. doi: 10.1128/JCM.39.12.4500-4505.2001.