PMID- 11724907
OWN - NLM
STAT- MEDLINE
DCOM- 20020117
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 49
IP  - 12
DP  - 2001 Dec
TI  - Immunolocalization of sonic hedgehog (Shh) in developing mouse lung.
PG  - 1593-604
AB  - Expression of sonic hedgehog (Shh) is required for normal development of the lung 
      during embryogenesis. Loss of Shh expression in mice results in tracheoesophageal 
      fistula, lung hypoplasia, and abnormal lung lobulation. To determine whether Shh 
      may play a role later in lung morphogenesis, immunostaining for Shh was performed 
      in mouse lung from embryonic day (E) 10.5 to postnatal day (PD) 24. Shh was 
      detected in the distal epithelium of the developing mouse lung from E10.5 to 
      E16.5. From E16.5 until PD15, Shh was present in epithelial cells in both the 
      peripheral and conducting airways. Although all cells of the developing 
      epithelium uniformly expressed Shh at E10.5, Shh expression was restricted to 
      subsets of epithelial cells by E16.5. Between E16.5 and PD15, non-uniform Shh 
      staining of epithelial cells was observed in the conducting airways in a pattern 
      consistent with the distribution of non-ciliated bronchiolar cells (i.e., Clara 
      cells) and the Clara cell marker CCSP. Shh did not co-localize with hepatocyte 
      nuclear factor/forkhead homologue-4 (HFH-4), beta-tubulin, or with the presence 
      of cilia. These results support the concept that Shh plays a distinct regulatory 
      role in the lung later in morphogenesis, when it may influence formation or 
      cytodifferentiation of the conducting airways.
FAU - Miller, L A
AU  - Miller LA
AD  - Children's Hospital Medical Center, Division of Pulmonary Biology, Cincinnati, 
      Ohio 45229-3039, USA.
FAU - Wert, S E
AU  - Wert SE
FAU - Whitsett, J A
AU  - Whitsett JA
LA  - eng
GR  - HL56387/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FOXI1 protein, human)
RN  - 0 (FOXJ1 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Hedgehog Proteins)
RN  - 0 (Proteins)
RN  - 0 (SCGB1A1 protein, human)
RN  - 0 (SHH protein, human)
RN  - 0 (Scgb1a1 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Tubulin)
RN  - 9060-09-7 (Uteroglobin)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - *DNA-Binding Proteins
MH  - Forkhead Transcription Factors
MH  - Hedgehog Proteins
MH  - Immunohistochemistry
MH  - Lung/embryology/*growth & development/*metabolism
MH  - Mice
MH  - Proteins/metabolism
MH  - Respiratory Mucosa/embryology/growth & development/metabolism
MH  - Trans-Activators/*metabolism
MH  - Tubulin/metabolism
MH  - *Uteroglobin
EDAT- 2001/11/29 10:00
MHDA- 2002/01/18 10:01
CRDT- 2001/11/29 10:00
PHST- 2001/11/29 10:00 [pubmed]
PHST- 2002/01/18 10:01 [medline]
PHST- 2001/11/29 10:00 [entrez]
AID - 10.1177/002215540104901213 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 2001 Dec;49(12):1593-604. doi: 10.1177/002215540104901213.