PMID- 11724927
OWN - NLM
STAT- MEDLINE
DCOM- 20020110
LR  - 20181113
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 98
IP  - 25
DP  - 2001 Dec 4
TI  - A caged Ab reveals an immediate/instructive effect of BDNF during hippocampal 
      synaptic potentiation.
PG  - 14702-7
AB  - Neurotrophins have been shown to be involved in functional strengthening of 
      central nervous system synapses. Although their general importance in this 
      process is undisputed, it remains unresolved whether neurotrophins are truly 
      mediators of synaptic strengthening or merely important cofactors. To address 
      this question, we have devised a method to inactivate endogenous brain-derived 
      neurotrophic factor (BDNF) with high time resolution by "caging" a 
      function-blocking mAb against BDNF with a photosensitive protecting compound. 
      Different assays were used to show that this inactivation of the Ab is reversible 
      by UV light. Synaptic potentiation after theta-burst [corrected] stimulation in 
      the CA1 region of acute hippocampal slices was significantly less when applying 
      the unmodified Ab compared with the caged Ab. Importantly, photoactivation of the 
      caged Ab during the time of induction of synaptic enhancement led to a marked 
      decrease in potentiation. Our experiments therefore strengthen the view that 
      endogenous BDNF has fast effects during induction of synaptic plasticity. The 
      results additionally show that caged Abs can provide a tool for precise 
      spatiotemporal control over endogenous protein levels.
FAU - Kossel, A H
AU  - Kossel AH
AD  - Max-Planck-Institut fur Neurobiologie, 82152 Munchen-Martinsried, Germany.
FAU - Cambridge, S B
AU  - Cambridge SB
FAU - Wagner, U
AU  - Wagner U
FAU - Bonhoeffer, T
AU  - Bonhoeffer T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20011127
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
EIN - Proc Natl Acad Sci U S A 2002 Jan 8;99(1):541
MH  - Animals
MH  - Antibodies, Monoclonal/chemistry/radiation effects
MH  - Brain-Derived Neurotrophic Factor/antagonists & inhibitors/immunology/*physiology
MH  - Female
MH  - Hippocampus/*physiology
MH  - In Vitro Techniques
MH  - Long-Term Potentiation/physiology
MH  - Mice
MH  - Photobiology
MH  - Time Factors
PMC - PMC64745
EDAT- 2001/11/29 10:00
MHDA- 2002/01/11 10:01
PMCR- 2002/06/04
CRDT- 2001/11/29 10:00
PHST- 2001/11/29 10:00 [pubmed]
PHST- 2002/01/11 10:01 [medline]
PHST- 2001/11/29 10:00 [entrez]
PHST- 2002/06/04 00:00 [pmc-release]
AID - 251326998 [pii]
AID - 3269 [pii]
AID - 10.1073/pnas.251326998 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14702-7. doi: 10.1073/pnas.251326998. 
      Epub 2001 Nov 27.