PMID- 11725084
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1423-0127 (Electronic)
IS  - 1021-7770 (Linking)
VI  - 3
IP  - 1
DP  - 1996 Jan
TI  - Characterization of Phospholipase A(2) Activation by Plasmin in Cultured Bovine 
      Endothelial Cells.
PG  - 59-66
AB  - Treatment of cultured bovine carotid artery endothelial cells with 0.1 &mgr;M 
      human plasmin has been reported to induce a receptor-mediated short burst of 
      arachidonate release, which is a pertussis toxin-sensitive and extracellular 
      calcium-dependent reaction. Plasmin-induced calcium influx in cells was 
      significantly inhibited by pretreatment with pertussis toxin, indicating that the 
      former was coupled with a pertussis toxin-sensitive guanosine 5'-triphosphate 
      (GTP)-binding protein. Plasmin significantly induced the formation of 
      lysophosphatidylcholine but not lysophosphatidylethanolamine. A cellular 
      phospholipase A(2) with an arachidonyl specificity at the sn-2 position of 
      phosphatidylcholine, which required submicromolar calcium, was identified as a 
      cytosolic phospholipase A(2) by immunoblot analysis. By a cell-free enzyme 
      activity assay and immunoblot analysis, plasmin was found to induce a 
      translocation of the cytosolic phospholipase A(2) from the cytosol to the 
      membrane. Taken together, the results suggest that plasmin bound to its putative 
      receptor and activated a GTP-binding protein coupled to calcium influx channel, 
      followed by translocation and activation of cytosolic phospholipase A(2) in 
      endothelial cells. Copyright 1996 S. Karger AG, Basel
FAU - Chang, W-C
AU  - Chang WC
AD  - Department of Pharmacology, National Cheng Kung University, Tainan, Taiwan, 
      Republic of China.
FAU - Shyu, W-J
AU  - Shyu WJ
FAU - Shi, G-Y
AU  - Shi GY
FAU - Lin, MT
AU  - Lin MT
FAU - Jen, CJ
AU  - Jen CJ
FAU - Wing, L-YC
AU  - Wing LY
FAU - Tang, M-J
AU  - Tang MJ
FAU - Wu, H-L
AU  - Wu HL
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Biomed Sci
JT  - Journal of biomedical science
JID - 9421567
EDAT- 1996/01/01 00:00
MHDA- 2001/11/29 10:00
CRDT- 1996/01/01 00:00
PHST- 1996/01/01 00:00 [pubmed]
PHST- 2001/11/29 10:00 [medline]
PHST- 1996/01/01 00:00 [entrez]
AID - jbs03059 [pii]
AID - 10.1007/BF02253580 [doi]
PST - ppublish
SO  - J Biomed Sci. 1996 Jan;3(1):59-66. doi: 10.1007/BF02253580.