PMID- 11725491
OWN - NLM
STAT- MEDLINE
DCOM- 20020429
LR  - 20181113
IS  - 1073-6085 (Print)
IS  - 1073-6085 (Linking)
VI  - 19
IP  - 2
DP  - 2001 Oct
TI  - Combining supercritical fluid extraction of soil herbicides with enzyme 
      immunoassay analysis.
PG  - 211-4
AB  - Supercritical fluid extraction (SFE) of soil herbicides followed by enzyme 
      immunoassay analysis (EIA) is explained in a step-by-step process. Extracted 
      herbicides, include 2,4-D, simazine, atrazine, and alachlor. The herbicide, 
      trifluralin was not successfully analyzed by EIA because of crossreacting 
      metabolites. Problems with SFE, including uneven packing of cells, leaks, uneven 
      flow and clogging, can largely be eliminated as the method parameters are 
      optimized. It was necessary to add modifiers including methanol or acetone to the 
      SF CO2 to increase the solubility of the analytes. Detection limits of 2.5 ng/g 
      soil for atrazine and alachlor and 15 ng/g soil for simazine and 2,4-D without 
      concentration of the sample were achieved. Recoveries above 80% and relative 
      standard deviations (RSDs) less than 15% for 2,4-D simazine, atrazine and 
      alachlor were achieved. Atrazine and alachlor recoveries were above 90% with RSDs 
      below 10%. Forty soil samples could be extracted and analyzed in an 8-h day.
FAU - Stearman, G K
AU  - Stearman GK
AD  - Center for the Management, Utilization and Protection of Water Resources, 
      Tennessee Technological University, Box 5033, Cookeville, TN 38505-0001, USA.
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Mol Biotechnol
JT  - Molecular biotechnology
JID - 9423533
RN  - 0 (Acetamides)
RN  - 0 (Herbicides)
RN  - 0 (Soil Pollutants)
RN  - 1364PS73AF (Acetone)
RN  - 24S2S61PXL (alachlor)
RN  - 2577AQ9262 (2,4-Dichlorophenoxyacetic Acid)
RN  - QJA9M5H4IM (Atrazine)
RN  - SG0C34SMY3 (Simazine)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - 2,4-Dichlorophenoxyacetic Acid/analysis
MH  - Acetamides/analysis
MH  - Acetone/pharmacology
MH  - Atrazine/analysis
MH  - Chromatography, Supercritical Fluid/*methods
MH  - Herbicides/*analysis
MH  - Immunoenzyme Techniques/*methods
MH  - Methanol/pharmacology
MH  - Reproducibility of Results
MH  - Simazine/analysis
MH  - Soil Pollutants
MH  - Time Factors
EDAT- 2001/12/01 10:00
MHDA- 2002/05/01 10:01
CRDT- 2001/12/01 10:00
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/05/01 10:01 [medline]
PHST- 2001/12/01 10:00 [entrez]
AID - MB:19:2:211 [pii]
AID - 10.1385/MB:19:2:211 [doi]
PST - ppublish
SO  - Mol Biotechnol. 2001 Oct;19(2):211-4. doi: 10.1385/MB:19:2:211.