PMID- 11726318
OWN - NLM
STAT- MEDLINE
DCOM- 20021002
LR  - 20061115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 86
IP  - 12
DP  - 2001 Dec
TI  - Fetal and embryonic hemoglobins in erythroblasts from fetal blood and fetal cells 
      enriched from maternal blood in fetal anemia.
PG  - 1270-6
AB  - BACKGROUND AND OBJECTIVES: To determine whether there is a delay or reversal in 
      switch mechanisms from embryonic (e and z) to fetal (g) hemoglobins accompanying 
      the erythroblastosis of anemic fetuses and whether an increased erythroblast 
      count in fetal blood is associated with an increase in feto-maternal cell 
      trafficking. DESIGN AND METHODS: Fetal and maternal blood samples were obtained 
      from 10 cases with rhesus isoimmunization and 2 cases with maternal Parvo-B19 
      virus at 19-33 weeks' gestation. Blood samples were also taken as controls from 
      61 fetuses and 86 mothers. Fetal erythroblasts were isolated by triple density 
      gradient centrifugation and magnetic cell sorting with CD71 antibody. Fluorescent 
      antibodies were used to immuno-stain for zeta (z), epsilon (e) and gamma (g) 
      hemoglobin chains. In the maternal samples, fluorescence in situ hybridization 
      (FISH) for X and Y chromosomes was also carried out to confirm the presence and 
      proportion of the enriched fetal cells from maternal blood. RESULTS: In both 
      fetal and maternal blood the percentage of erythroblasts positive for g-globin 
      chain was significantly higher in the anemic fetuses compared to the controls 
      (fetal blood, p<0.001, R=0.91; maternal blood, p<0.001, R=0.56), but there was no 
      significant difference in expression of the e and z-chains. The percentage of 
      cells with Y-signals was also higher in the maternal samples of anemic fetuses 
      compared to normal controls (p<0.001, R=0.56). INTERPRETATION AND CONCLUSIONS: 
      These findings suggest that the erythroblastosis of anemic fetuses is not 
      accompanied by a delay or a reversal in switch from embryonic to fetal hemoglobin 
      chains. Severe fetal anemia is associated with an increase in feto-maternal cell 
      trafficking.
FAU - Al-Mufti, R
AU  - Al-Mufti R
AD  - Harris Birthright Research Centre For Fetal Medicine, King's College Trust School 
      of Medicine, Denmark Hill, London SE5 8RX, England.
FAU - Hambley, H
AU  - Hambley H
FAU - Farzaneh, F
AU  - Farzaneh F
FAU - Nicolaides, K H
AU  - Nicolaides KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
RN  - 9004-22-2 (Globins)
RN  - 9034-63-3 (Fetal Hemoglobin)
SB  - IM
MH  - Adult
MH  - Anemia/*blood
MH  - Case-Control Studies
MH  - Cell Count
MH  - Erythroblasts/*chemistry/cytology
MH  - Female
MH  - Fetal Blood
MH  - Fetal Diseases/*blood
MH  - Fetal Hemoglobin/*analysis
MH  - Fetomaternal Transfusion/*blood
MH  - Globins/analysis
MH  - Humans
MH  - Pregnancy
EDAT- 2001/12/01 10:00
MHDA- 2002/10/03 04:00
CRDT- 2001/12/01 10:00
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/10/03 04:00 [medline]
PHST- 2001/12/01 10:00 [entrez]
PST - ppublish
SO  - Haematologica. 2001 Dec;86(12):1270-6.