PMID- 11727015
OWN - NLM
STAT- MEDLINE
DCOM- 20020129
LR  - 20161124
IS  - 0301-0449 (Print)
IS  - 0301-0449 (Linking)
VI  - 31
IP  - 12
DP  - 2001 Dec
TI  - Detection of early atherosclerosis with radiolabeled monocyte chemoattractant 
      protein-1 in prediabeteic Zucker rats.
PG  - 827-35
AB  - BACKGROUND: Migration of monocytes into the arterial wall is an early finding of 
      atherosclerosis. Monocytes are attracted to sites of vascular endothelial cell 
      injury, the initiating event in the development of atheromatous disease, by a 
      chemokine known as monocyte chemoattractant protein-1 (MCP-1). Injured vascular 
      endothelial and smooth muscle cells selectively secrete MCP-1. OBJECTIVE: This 
      study was performed to determine if radiolabeled MCP-1 would co-localize at sites 
      of monocyte/macrophage concentration in an experimental model of 
      transplant-induced vasculopathy in diabetic animals. MATERIALS AND METHODS: 
      Hearts from 3-month-old male Zucker rats, heterozygote (Lean) or homozygote (Fat) 
      for the diabetes-associated gene fa, were transplanted into the abdomens of 
      genetically matched recipients. Lean and Fat animals were then fed normal or 
      high-fat diets for 90 days. RESULTS: At 90 days significant increases (P < 0.013) 
      of MCP-1 graft uptake were seen at imaging and confirmed on scintillation gamma 
      well counting studies in Lean (n = 5) and Fat (n = 12) animals, regardless of 
      diet, 400 % and 40 %, above control values, respectively. MCP-1 uptake of native 
      and grafted hearts correlated with increased numbers of perivascular macrophages 
      (P < 0.02), as seen by immunostaining with an antibody specific for macrophages 
      (ED 2). CONCLUSION: Radiolabeled MCP-1 can detect abnormally increased numbers of 
      perivascular mononuclear cells in native and grafted hearts in prediabetic rats. 
      MCP-1 may be useful in the screening of diabetic children for early 
      atherosclerotic disease.
FAU - Blankenberg, F G
AU  - Blankenberg FG
AD  - Division of Pediatric Radiology, Lucile Salter Packard Children's Hospital, 
      Stanford, California, USA. blankenb@leland.stanford.edu
FAU - Wen, P
AU  - Wen P
FAU - Dai, M
AU  - Dai M
FAU - Zhu, D
AU  - Zhu D
FAU - Panchal, S N
AU  - Panchal SN
FAU - Tait, J F
AU  - Tait JF
FAU - Post, A M
AU  - Post AM
FAU - Strauss, H W
AU  - Strauss HW
FAU - Valantine, H A
AU  - Valantine HA
LA  - eng
GR  - HL47151/HL/NHLBI NIH HHS/United States
GR  - HL61717/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Pediatr Radiol
JT  - Pediatric radiology
JID - 0365332
RN  - 0 (Blood Glucose)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Blood Glucose
MH  - *Chemokine CCL2/pharmacokinetics
MH  - Coronary Artery Disease/*diagnostic imaging/immunology
MH  - Coronary Vessels/*diagnostic imaging/pathology
MH  - Diabetes Complications
MH  - Disease Models, Animal
MH  - Heart Transplantation/*diagnostic imaging/immunology
MH  - Immunohistochemistry
MH  - Leukocytes, Mononuclear/diagnostic imaging
MH  - Male
MH  - Radionuclide Imaging
MH  - Rats
MH  - Rats, Zucker
EDAT- 2001/12/01 10:00
MHDA- 2002/01/30 10:01
CRDT- 2001/12/01 10:00
PHST- 2000/10/11 00:00 [received]
PHST- 2001/05/21 00:00 [accepted]
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/01/30 10:01 [medline]
PHST- 2001/12/01 10:00 [entrez]
AID - 10.1007/s002470100000 [doi]
PST - ppublish
SO  - Pediatr Radiol. 2001 Dec;31(12):827-35. doi: 10.1007/s002470100000.