PMID- 11727324
OWN - NLM
STAT- MEDLINE
DCOM- 20011227
LR  - 20051116
IS  - 1367-6733 (Print)
IS  - 1367-6733 (Linking)
VI  - 4
IP  - 1
DP  - 2001 Jan
TI  - Prediction of drug allergenicity: possible use of the local lymph node assay.
PG  - 60-5
AB  - The mouse local lymph node assay (LLNA) is currently used for the prospective 
      identification of chemicals that have the potential to cause skin sensitization 
      and allergic contact dermatitis. In this respect, the assay has been fully and 
      formally evaluated and validated, and has been accepted recently as a stand-alone 
      method for the identification of potential skin sensitizers. The assay involves 
      topical application of test substance and the subsequent measurement of 
      proliferative responses in the lymph nodes draining the site of exposure. The 
      testing of new drug entities using a similar assay technique could offer a 
      potential alternative for the identification of potential drug allergens. 
      Currently, the popliteal lymph node assay (PLNA), or modifications of it, are 
      used in research studies for the identification of drugs which have the potential 
      to cause allergic or autoimmunogenic reactions. The PLNA involves subcutaneous 
      application of test substance into the hind footpad, followed by the measurement 
      of proliferative responses, or other parameters of immune activation, in the 
      popliteal lymph nodes. However, these assays have not been validated 
      systematically and the potential utility of a modified LLNA for use in the 
      identification of such compounds is discussed.
FAU - Warbrick, E V
AU  - Warbrick EV
AD  - Zeneca Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, SK10 
      4TJ, UK. Vicky.Warbrick@syngenta.com
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Curr Opin Drug Discov Devel
JT  - Current opinion in drug discovery & development
JID - 100887519
SB  - IM
MH  - Animals
MH  - Biological Assay/*methods
MH  - Dermatitis, Contact/*etiology/immunology
MH  - Drug Hypersensitivity/*etiology/immunology
MH  - Humans
MH  - Lymph Nodes/*drug effects/*immunology
MH  - Mice
MH  - Predictive Value of Tests
RF  - 45
EDAT- 2001/12/01 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/12/01 10:00
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/12/01 10:00 [entrez]
PST - ppublish
SO  - Curr Opin Drug Discov Devel. 2001 Jan;4(1):60-5.