PMID- 11728452
OWN - NLM
STAT- MEDLINE
DCOM- 20011226
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 508
IP  - 3
DP  - 2001 Nov 23
TI  - NF-kappaB is required for TNF-alpha-directed smooth muscle cell migration.
PG  - 360-4
AB  - Migration of vascular smooth muscle cells (VSMC) is a crucial event in the 
      formation of vascular stenotic lesions. Tumor necrosis factor-alpha (TNF-alpha) 
      is elaborated by VSMC in atherosclerosis and following angioplasty. We 
      investigated the role of nuclear factor-kappaB (NF-kappaB) in human VSMC 
      migration induced by TNF-alpha. Adenoviral expression of a mutant form of the 
      inhibitor of NF-kappaB, IkappaB-alphaM, suppressed TNF-alpha-triggered 
      degradation of cellular IkappaB-alpha, inhibited activation of NF-kappaB, and 
      attenuated TNF-alpha-induced migration. Further, IkappaB-alphaM suppressed 
      TNF-alpha-stimulated release of interleukin-6 and -8 (IL-6 and IL-8). 
      Neutralization of IL-6 and IL-8 with appropriate antibodies reduced 
      TNF-alpha-induced VSMC migration. Addition of recombinant IL-6 and IL-8 
      stimulated migration. Collectively, our data provide initial evidence that 
      TNF-alpha-mediated VSMC migration requires NF-kappaB activation and is associated 
      with induction of IL-6 and IL-8 which act in an autocrine manner.
FAU - Wang, Z
AU  - Wang Z
AD  - Division of Basic Medical Science, Mercer University School of Medicine, Macon, 
      GA 31207, USA. wang_z@mercer.edu
FAU - Castresana, M R
AU  - Castresana MR
FAU - Newman, W H
AU  - Newman WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (NFKBIA protein, human)
RN  - 0 (Nucleic Acid Synthesis Inhibitors)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Autocrine Communication
MH  - Cell Movement
MH  - Cells, Cultured
MH  - *Chemotaxis
MH  - Cycloheximide/pharmacology
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Dactinomycin/pharmacology
MH  - Genetic Vectors
MH  - Humans
MH  - *I-kappa B Proteins
MH  - Interleukin-6/genetics/metabolism/pharmacology
MH  - Interleukin-8/genetics/metabolism/pharmacology
MH  - Muscle, Smooth, Vascular/*cytology/*metabolism
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*metabolism
MH  - Nucleic Acid Synthesis Inhibitors/pharmacology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Proteins/metabolism/pharmacology
MH  - Tumor Necrosis Factor-alpha/*pharmacology/physiology
EDAT- 2001/12/01 10:00
MHDA- 2002/01/05 10:01
CRDT- 2001/12/01 10:00
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/12/01 10:00 [entrez]
AID - S0014-5793(01)03109-X [pii]
AID - 10.1016/s0014-5793(01)03109-x [doi]
PST - ppublish
SO  - FEBS Lett. 2001 Nov 23;508(3):360-4. doi: 10.1016/s0014-5793(01)03109-x.