PMID- 11729181
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 5
DP  - 2002 Feb 1
TI  - Phorbol 12-myristate 13-acetate inhibits death receptor-mediated apoptosis in 
      Jurkat cells by disrupting recruitment of Fas-associated polypeptide with death 
      domain.
PG  - 3776-83
AB  - Regulation of death receptor-mediated apoptosis is incompletely understood. 
      Previous studies have demonstrated that phorbol 12-myristate 13-acetate (PMA), a 
      protein kinase C activator, inhibits Fas (CD95)-mediated apoptosis in Jurkat 
      (type II) cells but not SKW6.4 (type I) cells. In this study, we demonstrated 
      that PMA also protects Jurkat cells from apoptosis induced by tumor necrosis 
      factor-alpha and the tumor necrosis factor-alpha-related apoptosis-inducing 
      ligand (TRAIL). Interestingly, PMA failed to protect Jurkat cells from apoptosis 
      induced by other agents, including etoposide, camptothecin, and 
      gamma-irradiation. Analysis of the initial events induced by agonistic anti-Fas 
      antibodies revealed that PMA inhibited Fas binding to Fas-associated polypeptide 
      with death domain (FADD) in Jurkat cells but not in SKW6.4 cells. Although the 
      protein kinase inhibitor bisindoylmaleimide VIII increased apoptosis induced by 
      agonistic anti-Fas antibody, tumor necrosis factor-alpha, and TRAIL, these 
      effects were not observed with the protein kinase C inhibitor H7 and were not 
      associated with increased FADD recruitment to Fas. These results indicate that 
      PMA inhibits death signaling induced by a number of discrete receptors and 
      suggest that the effects are mediated at the level of receptor-mediated adaptor 
      molecule recruitment.
FAU - Meng, Xue Wei
AU  - Meng XW
AD  - Division of Oncology Research, Department of Molecular Pharmacology, Mayo Clinic, 
      Mayo Graduate School, Rochester, Minnesota 55905, USA.
FAU - Heldebrant, Michael P
AU  - Heldebrant MP
FAU - Kaufmann, Scott H
AU  - Kaufmann SH
LA  - eng
GR  - R01 CA069008/CA/NCI NIH HHS/United States
GR  - R01 CA69008/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20011129
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, CD)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (FADD protein, human)
RN  - 0 (Fas-Associated Death Domain Protein)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFRSF10A protein, human)
RN  - 0 (TNFSF10 protein, human)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (fas Receptor)
RN  - 6PLQ3CP4P3 (Etoposide)
RN  - 98600C0908 (Cycloheximide)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - XT3Z54Z28A (Camptothecin)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Antigens, CD/drug effects/physiology
MH  - Apoptosis/*drug effects/radiation effects
MH  - Apoptosis Regulatory Proteins
MH  - Binding Sites
MH  - Camptothecin/toxicity
MH  - Carrier Proteins/*antagonists & inhibitors
MH  - Cell Line
MH  - Cycloheximide/pharmacology
MH  - Etoposide/toxicity
MH  - Fas-Associated Death Domain Protein
MH  - Gamma Rays
MH  - HSP90 Heat-Shock Proteins/physiology
MH  - Humans
MH  - Jurkat Cells
MH  - Kinetics
MH  - Membrane Glycoproteins/pharmacology
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand
MH  - Receptors, Tumor Necrosis Factor/*antagonists & inhibitors
MH  - TNF-Related Apoptosis-Inducing Ligand
MH  - Tetradecanoylphorbol Acetate/*pharmacology
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - fas Receptor/drug effects/*physiology
EDAT- 2001/12/01 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/12/01 10:00
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/12/01 10:00 [entrez]
AID - S0021-9258(20)87673-X [pii]
AID - 10.1074/jbc.M107218200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Feb 1;277(5):3776-83. doi: 10.1074/jbc.M107218200. Epub 2001 
      Nov 29.