PMID- 11729350
OWN - NLM
STAT- MEDLINE
DCOM- 20020110
LR  - 20180213
IS  - 1018-2438 (Print)
IS  - 1018-2438 (Linking)
VI  - 126
IP  - 2
DP  - 2001 Oct
TI  - Prolonged antigen exposure ameliorates airway inflammation but not remodeling in 
      a mouse model of bronchial asthma.
PG  - 126-34
AB  - BACKGROUND: In naive rodents, repeated exposure to aerosolized antigen induces 
      suppression of the Th2 response to the antigen. We hypothesized that more 
      prolonged exposure of established asthma model to antigen aerosols may 
      downregulate asthmatic phenotype. METHODS: After establishing an ovalbumin 
      (OVA)-induced asthma model, mice were further exposed to OVA (prolonged exposure 
      group) or phosphate-buffered saline (positive controls) 3 days per week for 6 
      weeks. During week 7, the mice of both groups were finally challenged with OVA. 
      RESULTS: Prolonged OVA exposure resulted in marked suppression of serum 
      OVA-specific immunoglobulin E (IgE) antibody levels, eosinophilia of the airway, 
      and airway hyperresponsiveness (AHR). However, airway remodeling characterized by 
      goblet cell hyperplasia and airway fibrosis was observed to the same degree in 
      both groups. These effects were accompanied by diminished production of Th2 
      cytokines such as interleukin-4 (IL-4), IL-5 and IL-13 in bronchoalveolar lavage 
      fluid (BALF) and cultured supernatant of splenocytes. Furthermore, prolonged 
      exposure markedly increased IL-12 levels in BALF. CONCLUSIONS: Prolonged antigen 
      exposure has inhibitory effects on eosinophilic inflammation, AHR and IgE 
      response to antigen, but not on airway remodeling, presumably via inhibition of 
      Th2 cytokines and increased IL-12 production in the lungs.
CI  - Copyright 2001 S. Karger AG, Basel
FAU - Sakai, K
AU  - Sakai K
AD  - Second Department of Internal Medicine, Ehime University, Onsen-gun, Ehime, 
      Japan.
FAU - Yokoyama, A
AU  - Yokoyama A
FAU - Kohno, N
AU  - Kohno N
FAU - Hamada, H
AU  - Hamada H
FAU - Hiwada, K
AU  - Hiwada K
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Antigens)
RN  - 0 (Cytokines)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Antibody Specificity
MH  - Antigens/administration & dosage/*immunology
MH  - Asthma/immunology/*physiopathology
MH  - Bronchial Hyperreactivity/immunology
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Cytokines/biosynthesis
MH  - Disease Models, Animal
MH  - Female
MH  - Germ-Free Life
MH  - Humans
MH  - *Immune Tolerance
MH  - Immunoglobulin E/blood
MH  - Inflammation/immunology/*physiopathology
MH  - Lung/immunology/pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/administration & dosage/*immunology
MH  - Respiratory System/*immunology
EDAT- 2001/12/01 10:00
MHDA- 2002/01/11 10:01
CRDT- 2001/12/01 10:00
PHST- 2001/12/01 10:00 [pubmed]
PHST- 2002/01/11 10:01 [medline]
PHST- 2001/12/01 10:00 [entrez]
AID - iaa26126 [pii]
AID - 10.1159/000049503 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2001 Oct;126(2):126-34. doi: 10.1159/000049503.