PMID- 11730483
OWN - NLM
STAT- MEDLINE
DCOM- 20020402
LR  - 20191105
IS  - 0885-4513 (Print)
IS  - 0885-4513 (Linking)
VI  - 34
IP  - 3
DP  - 2001 Dec
TI  - Efficient cryopreservation of dendritic cells transfected with cDNA of a tumour 
      antigen for clinical application.
PG  - 161-6
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells of the immune 
      system and are currently being investigated in clinical applications as cancer 
      vaccines. An efficient cryopreservation method would greatly contribute to their 
      use in clinical trials. We have established a method for freezing of DCs derived 
      from peripheral blood mononuclear cells using the plasma expander Gelifundol. 
      This enabled us to reduce the concentration of the toxic DMSO to 5%. The method 
      could be performed without the addition of fetal calf serum or any other serum. 
      After freezing, the viability of the DCs was 90%. The cells exhibited all the 
      phenotypic characteristics (CD11c+, HLA-DR+, CD80+, CD83+, CD86+) of DCs, as 
      tested by flow cytometry. Cells transfected with cDNA for the tumour antigen 
      mucin expressed this protein on their surfaces in the same manner as before 
      freezing. The stimulating capacity of a mixed lymphocyte culture was also 
      preserved. These findings offer an efficient method for the cryopreservation of 
      DCs for use in clinical trials.
FAU - Pecher, G
AU  - Pecher G
AD  - Humboldt-University Berlin, Charite Campus Mitte, AG Molecular Gene- and 
      Immunotherapy, Hessische Str. 3-4, 10115 Berlin, Germany. 
      gabriele.pecher@charite.de
FAU - Schirrmann, T
AU  - Schirrmann T
FAU - Kaiser, L
AU  - Kaiser L
FAU - Schenk, J A
AU  - Schenk JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cancer Vaccines)
RN  - 0 (DNA, Complementary)
RN  - 0 (Mucins)
SB  - IM
MH  - Antigens, Neoplasm/genetics/*physiology
MH  - Cancer Vaccines
MH  - Cell Division/physiology
MH  - Cell Survival/physiology
MH  - Cryopreservation/*methods
MH  - DNA, Complementary/genetics
MH  - Dendritic Cells/immunology/*physiology
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Immunophenotyping
MH  - Mucins/immunology
MH  - Transfection
EDAT- 2001/12/04 10:00
MHDA- 2002/04/03 10:01
CRDT- 2001/12/04 10:00
PHST- 2001/12/04 10:00 [pubmed]
PHST- 2002/04/03 10:01 [medline]
PHST- 2001/12/04 10:00 [entrez]
AID - 10.1042/ba20010034 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2001 Dec;34(3):161-6. doi: 10.1042/ba20010034.