PMID- 11730564
OWN - NLM
STAT- MEDLINE
DCOM- 20031104
LR  - 20141120
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 22
IP  - 1
DP  - 2001 Jan
TI  - Effects of calcitriol and its analogues on interaction of MCP-1 and monocyte 
      derived dendritic cells in vitro.
PG  - 62-5
AB  - AIM: To study the effect of calcitriol [1,25(OH)2D3] and its analogues on the 
      interaction of monocyte chemoattractant protein-1 (MCP-1) and in vitro generated 
      monocyte-derived dendritic cells (MoDC). METHODS: MoDC were obtained by 
      differentiating monocytes in exposure to GM-CSF and IL-4 for 5 d. mRNA expression 
      of MCP-1 and its receptors were analyzed by RT-PCR, and protein production of 
      MCP-1 by ELISA and migratory ability of MoDC in response to MCP-1 by a 
      micromultiwell chemotaxis chamber assay. RESULTS: MoDC can express MCP-1 mRNA, 
      and secret a low level of MCP-1 protein and has the ability to migrate to MCP-1 
      in corresponding to its expression of MCP-1 receptors. Calcitriol and its 
      analogues with the same affinity to vitamin D receptor up-regulated the gene 
      expression of both MCP-1 and its receptors, enhanced MCP-1 protein production and 
      promoted the migratory ability of MoDC to MCP-1. CONCLUSION: The interaction of 
      DC and MCP-1 found in this study may suggest a possible auto-regulatory role 
      between DC and MCP-1 and the modulatory effect of calcitriol and its analogues on 
      DC and MCP-1 might provide an understanding of their positive role in tumors.
FAU - Zhu, K J
AU  - Zhu KJ
AD  - Department of Dermatology, 2nd Affiliated Hospital of Zhejiang University, School 
      of Medicine, Hangzhou 310009, China. zhukejian@mail.hz.zj.cn
FAU - Shen, Q Y
AU  - Shen QY
FAU - Zheng, M
AU  - Zheng M
FAU - Mrowietz, U
AU  - Mrowietz U
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Dihydroxycholecalciferols)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 143NQ3779B (calcipotriene)
RN  - 60965-80-2 (1 alpha,24-dihydroxyvitamin D3)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Calcitriol/*analogs & derivatives/*pharmacology
MH  - Cell Movement/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Dendritic Cells/*drug effects/metabolism
MH  - Dihydroxycholecalciferols/pharmacology
MH  - Humans
MH  - RNA, Messenger/biosynthesis/drug effects/genetics
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/biosynthesis/genetics
EDAT- 2001/12/04 10:00
MHDA- 2003/11/05 05:00
CRDT- 2001/12/04 10:00
PHST- 2001/12/04 10:00 [pubmed]
PHST- 2003/11/05 05:00 [medline]
PHST- 2001/12/04 10:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2001 Jan;22(1):62-5.