PMID- 11730565
OWN - NLM
STAT- MEDLINE
DCOM- 20031104
LR  - 20171116
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 22
IP  - 1
DP  - 2001 Jan
TI  - Gene expression of monocyte chemoattractant protein-1 in human monocytes by 
      exposure to advanced glycosylation end products.
PG  - 67-70
AB  - AIM: To explore the effects of advanced glycosylation end products (AGEP) on 
      monocyte chemoattractant protein-1 (MCP-1) gene expression in human peripheral 
      blood monocytes/macrophages (PBMC). METHODS: Expression of MCP-1 mRNA in PBMC 
      incubated with AGEP-bovine serum albumin (AGEP-BSA) was examined by 
      semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) with 
      beta-actin as an internal standard. Sequencing of RT-PCR products was performed 
      to confirm the specificity of amplification for MCP-1 gene. RESULTS: AGEP-BSA 
      stimulated monocytes to express MCP-1 mRNA in a glucose-concentration-related 
      fashion. The levels of MCP-1 mRNA were increased slightly when monocytes were 
      exposed to AGEP-BSA 200 mg/L (glycosylated with glucose 20 mmol/L), and increased 
      markedly when exposed to AGEP-BSA 200 mg/L (glycosylated with glucose 50 mmol/L), 
      but decreased slightly when exposed to AGEP-BSA 200 mg/L (glycosylated with 
      glucose 80 mmol/L). Expression of MCP-1 mRNA was undetectable in freshly isolated 
      monocytes, but was induced at 12 h and reached a maximal level at 24 h and was 
      almost undetectable at 36 h after the monocytes were incubated with AGEP-BSA 200 
      mg/L (P < 0.01). CONCLUSION: AGEP enhanced MCP-1 mRNA expression in human PBMC.
FAU - Wang, B R
AU  - Wang BR
AD  - Division of Cardiology, The Zhong-Da Hospital and School of Clinical Medicine, 
      Southeast University, Nanjing 210009, China.
FAU - Liu, N F
AU  - Liu NF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Gene Expression/*drug effects
MH  - Glycation End Products, Advanced/*pharmacology
MH  - Humans
MH  - Monocytes/*drug effects/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2001/12/04 10:00
MHDA- 2003/11/05 05:00
CRDT- 2001/12/04 10:00
PHST- 2001/12/04 10:00 [pubmed]
PHST- 2003/11/05 05:00 [medline]
PHST- 2001/12/04 10:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2001 Jan;22(1):67-70.