PMID- 11730855
OWN - NLM
STAT- MEDLINE
DCOM- 20020201
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 259
IP  - 1-2
DP  - 2002 Jan 1
TI  - Engineered expression of the Coxsackie B and adenovirus receptor (CAR) in human 
      dendritic cells enhances recombinant adenovirus-mediated gene transfer.
PG  - 205-15
AB  - Dendritic cells (DCs) are key antigen-presenting cells (APCs) that act as central 
      modulators of cellular immune responses. Genetic modification of DCs has 
      considerable therapeutic potential in the treatment of a wide spectrum of 
      diseases, including cancer and persistent viral infection. In this report, we 
      show that pre-treatment of DCs with a recombinant adenovirus encoding the major 
      adenovirus receptor, Coxsackie B and adenovirus receptor (CAR), significantly 
      increased the uptake of recombinant adenoviruses (Ads) by primary immature 
      monocyte-derived DCs. This could be correlated with CAR mRNA and surface protein 
      expression. Transduction of DCs by recombinant adenoviruses did not significantly 
      alter cellular viability. Therefore, we propose that pre-treatment of DCs with 
      Ad5-CAR is one strategy to increase the susceptibility of DCs to transduction by 
      recombinant Ads.
FAU - Stockwin, Luke H
AU  - Stockwin LH
AD  - School of Biochemistry and Molecular Biology, Mount Preston Street, University of 
      Leeds, Leeds LS2 9JT, UK.
FAU - Matzow, Torkjel
AU  - Matzow T
FAU - Georgopoulos, Nikolaos T
AU  - Georgopoulos NT
FAU - Stanbridge, Lindsay J
AU  - Stanbridge LJ
FAU - Jones, Samantha V
AU  - Jones SV
FAU - Martin, Iain G
AU  - Martin IG
FAU - Blair-Zajdel, Maria E
AU  - Blair-Zajdel ME
FAU - Blair, G Eric
AU  - Blair GE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Receptors, Virus)
RN  - 0 (adenovirus receptor)
RN  - 0 (coxsackievirus B receptor)
SB  - IM
MH  - Antigen Presentation/*genetics/immunology
MH  - Dendritic Cells/*immunology
MH  - Humans
MH  - Reassortant Viruses/*genetics/immunology
MH  - Receptors, Virus/*genetics/immunology
MH  - Transfection/methods
EDAT- 2001/12/04 10:00
MHDA- 2002/02/02 10:01
CRDT- 2001/12/04 10:00
PHST- 2001/12/04 10:00 [pubmed]
PHST- 2002/02/02 10:01 [medline]
PHST- 2001/12/04 10:00 [entrez]
AID - S0022175901005105 [pii]
AID - 10.1016/s0022-1759(01)00510-5 [doi]
PST - ppublish
SO  - J Immunol Methods. 2002 Jan 1;259(1-2):205-15. doi: 
      10.1016/s0022-1759(01)00510-5.