PMID- 11732850
OWN - NLM
STAT- MEDLINE
DCOM- 20020129
LR  - 20190607
IS  - 0018-0661 (Print)
IS  - 0018-0661 (Linking)
VI  - 134
IP  - 2
DP  - 2001
TI  - Physical mapping of 45S and 5S rDNA on maize metaphase and sorted chromosomes by 
      FISH.
PG  - 141-5
AB  - Physical locations of 45S and 5S rDNA were detected in maize by fluorescence in 
      situ hybridization (FISH). The FISH results on metaphase chromosome spreads 
      showed the 45S rDNA was located just at the nucleolus organizer region (NOR) on 
      6S (the short arm) of chromosome 6) as expected, and 5S rDNA at the distal region 
      of 2L (the long arm of chromosome 2). No signals were detected on the other maize 
      chromosomes for these two probes. The precision of the chromosomal position of a 
      hybridization site is related to the number of measurements. We also mapped the 
      location of 5S rDNA at the same sites on 2L by FISH on sorted chromosomes. We 
      could calculate more precisely the percentage distance of hybridization signals 
      since we had large numbers of target chromosomes on a small spot on the slide by 
      flow sorting. The percentage distance from centromere to the hybridization site 
      was 85% for 5S rDNA on 2L. The physical location of 5S rDNA was inconsistent with 
      its genetic site, which was positioned in the middle of genetic linkage group 2. 
      A FISH procedure for mapping genes on sorted plant chromosomes is described and 
      occurrence of only one 45S or 5S rDNA site on maize genome is discussed.
FAU - Li, L
AU  - Li L
AD  - Department of Agronomy, University of Nebraska, Lincoln 68588-0665, USA.
FAU - Arumuganathan, K
AU  - Arumuganathan K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 0 (RNA, ribosomal, 45S)
SB  - IM
MH  - Centromere
MH  - Chimera/genetics
MH  - Chromosome Mapping
MH  - Flow Cytometry
MH  - Genetic Linkage
MH  - Karyotyping
MH  - Nucleolus Organizer Region/genetics
MH  - RNA, Ribosomal/*genetics
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Zea mays/*genetics
EDAT- 2001/12/06 10:00
MHDA- 2002/01/30 10:01
CRDT- 2001/12/06 10:00
PHST- 2001/12/06 10:00 [pubmed]
PHST- 2002/01/30 10:01 [medline]
PHST- 2001/12/06 10:00 [entrez]
AID - 10.1111/j.1601-5223.2001.00141.x [doi]
PST - ppublish
SO  - Hereditas. 2001;134(2):141-5. doi: 10.1111/j.1601-5223.2001.00141.x.