PMID- 11733348
OWN - NLM
STAT- MEDLINE
DCOM- 20011218
LR  - 20191210
IS  - 0002-9440 (Print)
IS  - 1525-2191 (Electronic)
IS  - 0002-9440 (Linking)
VI  - 159
IP  - 6
DP  - 2001 Dec
TI  - BRD4 bromodomain gene rearrangement in aggressive carcinoma with translocation 
      t(15;19).
PG  - 1987-92
AB  - Translocation t(15;19)(q13;p13.1) defines a lethal midline carcinoma arising 
      adjacent to respiratory tract in young people. To characterize molecular 
      alterations responsible for the distinctly aggressive biological behavior of this 
      cancer, we mapped the chromosome 15 and 19 translocation breakpoints by 
      fluorescence in situ hybridization (FISH) and Southern blotting. To evaluate 
      preliminarily the frequency, anatomical distribution, and histological features 
      of t(15;19) cancer, we developed a FISH assay for paraffin sections. Our findings 
      reveal a novel oncogenic mechanism in which the chromosome 19 translocation 
      breakpoint interrupts the coding sequence of a bromodomain gene, BRD4. These 
      studies implicate BRD4 as a potential partner in a t(15;19)-associated fusion 
      oncogene. In addition, we localized the chromosome 15 breakpoint to a 9-kb region 
      in each of two cases, thereby identifying several candidate oncogenes which might 
      represent the BRD4 fusion partner. FISH evaluation of 13 pediatric carcinomas 
      revealed t(15;19) in one of four sinonasal carcinomas, whereas this translocation 
      was not detected in thymic (n = 3), mucoepidermoid (n = 3), laryngeal (n = 2), or 
      nasopharyngeal (n = 1) carcinomas. Our studies shed light on the oncogenic 
      mechanism underlying t(15;19) and provide further evidence that this highly 
      lethal cancer arises from respiratory mucosa.
FAU - French, C A
AU  - French CA
AD  - Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 
      02115, USA. cafrench@bics.bwh.harvard.edu
FAU - Miyoshi, I
AU  - Miyoshi I
FAU - Aster, J C
AU  - Aster JC
FAU - Kubonishi, I
AU  - Kubonishi I
FAU - Kroll, T G
AU  - Kroll TG
FAU - Dal Cin, P
AU  - Dal Cin P
FAU - Vargas, S O
AU  - Vargas SO
FAU - Perez-Atayde, A R
AU  - Perez-Atayde AR
FAU - Fletcher, J A
AU  - Fletcher JA
LA  - eng
SI  - GENBANK/AF386649
GR  - T32-HLO7627/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (BRD4 protein, human)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (Protein Isoforms)
RN  - 0 (Transcription Factors)
SB  - IM
CIN - Am J Pathol. 2001 Dec;159(6):1979-80. PMID: 11733346
MH  - Adolescent
MH  - Adult
MH  - Alternative Splicing
MH  - Blotting, Southern
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Cell Cycle Proteins
MH  - Child
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 15/*genetics
MH  - Chromosomes, Human, Pair 19/*genetics
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Gene Rearrangement/*genetics
MH  - Genes/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Nuclear Proteins
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Protein Isoforms/genetics
MH  - Transcription Factors
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
PMC - PMC1850578
EDAT- 2001/12/26 10:00
MHDA- 2002/01/05 10:01
PMCR- 2002/06/01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/01/05 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
PHST- 2002/06/01 00:00 [pmc-release]
AID - S0002-9440(10)63049-0 [pii]
AID - 2953 [pii]
AID - 10.1016/S0002-9440(10)63049-0 [doi]
PST - ppublish
SO  - Am J Pathol. 2001 Dec;159(6):1987-92. doi: 10.1016/S0002-9440(10)63049-0.