PMID- 11733499
OWN - NLM
STAT- MEDLINE
DCOM- 20020321
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 277
IP  - 7
DP  - 2002 Feb 15
TI  - Accurate chromatin organization of the mouse mammary tumor virus promoter 
      determines the nature of the synergism between transcription factors.
PG  - 4911-7
AB  - The mechanism underlying the synergism between transcription factors in 
      eukaryotic gene expression is not fully understood. In minichromosomes assembled 
      in vitro the synergism between steroid hormone receptors (SHRs) and nuclear 
      factor 1 (NF1) on the mouse mammary tumor virus (MMTV) promoter does not require 
      the proline-rich transactivation domain (PRD) of NF1. Here we show that similar 
      results are obtained in yeast. In contrast, replacing the native 
      hormone-responsive elements (HREs) by a single HRE results in a more accessible 
      chromatin and makes the synergism with SHR dependent on the PRD of NF1. Following 
      hormone induction, in addition to glucocorticoid receptor, the DNA binding domain 
      of NF1 is needed and sufficient for establishing an open chromatin conformation 
      on the wild type MMTV promoter. Thus, NF1 acts as a classical transcription 
      factor in a relaxed chromatin context, whereas in the context of the wild type 
      chromatin DNA binding of NF1 is sufficient to cooperate with SHRs by stabilizing 
      an open chromatin conformation.
FAU - Prado, Felix
AU  - Prado F
AD  - Institut fur Molekularbiologie und Tumorforschung (IMT), Philipps-Universitat, 
      E-Mannkopff-Str. 2, D-35033 Marburg, Germany.
FAU - Koop, Ronald
AU  - Koop R
FAU - Beato, Miguel
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20011130
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromatin)
RN  - 0 (Neurofibromin 1)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
RN  - EC 3.1.21.4 (GANTC-specific type II deoxyribonucleases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Chromatin/*chemistry/*metabolism
MH  - Chromosomes/metabolism
MH  - DNA/metabolism
MH  - Deoxyribonuclease I/metabolism
MH  - Deoxyribonucleases, Type II Site-Specific/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mutation
MH  - Neurofibromin 1/chemistry/*metabolism
MH  - Plasmids/metabolism
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - Protein Conformation
MH  - Protein Structure, Tertiary
MH  - Rats
MH  - Swine
MH  - Time Factors
MH  - Transcription Factors/*metabolism
MH  - Transcription, Genetic
MH  - Transcriptional Activation
MH  - beta-Galactosidase/metabolism
EDAT- 2001/12/26 10:00
MHDA- 2002/03/22 10:01
CRDT- 2001/12/26 10:00
PHST- 2001/12/26 10:00 [pubmed]
PHST- 2002/03/22 10:01 [medline]
PHST- 2001/12/26 10:00 [entrez]
AID - S0021-9258(19)82516-4 [pii]
AID - 10.1074/jbc.M110094200 [doi]
PST - ppublish
SO  - J Biol Chem. 2002 Feb 15;277(7):4911-7. doi: 10.1074/jbc.M110094200. Epub 2001 
      Nov 30.