PMID- 11734213
OWN - NLM
STAT- MEDLINE
DCOM- 20020122
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 509
IP  - 1
DP  - 2001 Nov 30
TI  - Overexpression of hepatocyte growth factor/scatter factor promotes 
      vascularization and granulation tissue formation in vivo.
PG  - 95-100
AB  - The effect of hepatocyte growth factor/scatter factor (HGF/SF) during wound 
      healing in the skin was investigated, using HGF/SF-overexpressing transgenic 
      mouse model. Histological analysis of HGF/SF transgenic mouse excisional wound 
      sites revealed increased granulation tissue with marked vascularization. Northern 
      blot analysis demonstrated that, relative to control, vascular endothelial growth 
      factor (VEGF) expression in transgenic skin was significantly higher at baseline 
      and was robustly up-regulated during wound healing. Elevated levels of VEGF 
      protein were detected immunohistochemically, predominantly in endothelial cells 
      and fibroblasts within the granulation tissue of HGF/SF transgenic skin. Serum 
      levels of VEGF were also elevated in HGF/SF transgenic mice. Thus, results from 
      our study suggest that HGF/SF has a significant effect on vascularization and 
      granulation tissue formation during wound healing in vivo, involving with 
      induction of VEGF.
FAU - Toyoda, M
AU  - Toyoda M
AD  - First Department of Internal Medicine, Gunma University School of Medicine, 
      Japan.
FAU - Takayama, H
AU  - Takayama H
FAU - Horiguchi, N
AU  - Horiguchi N
FAU - Otsuka, T
AU  - Otsuka T
FAU - Fukusato, T
AU  - Fukusato T
FAU - Merlino, G
AU  - Merlino G
FAU - Takagi, H
AU  - Takagi H
FAU - Mori, M
AU  - Mori M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA, Complementary)
RN  - 0 (Endothelial Growth Factors)
RN  - 0 (Lymphokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (Vascular Endothelial Growth Factors)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - DNA, Complementary/metabolism
MH  - Endothelial Growth Factors/*biosynthesis/blood
MH  - Endothelium/metabolism
MH  - Fibroblasts/metabolism
MH  - Hepatocyte Growth Factor/*biosynthesis/*physiology
MH  - Immunohistochemistry
MH  - Lymphokines/*biosynthesis/blood
MH  - Mice
MH  - Mice, Transgenic
MH  - *Neovascularization, Pathologic
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Time Factors
MH  - Vascular Endothelial Growth Factor A
MH  - Vascular Endothelial Growth Factors
MH  - Wound Healing
EDAT- 2001/12/06 10:00
MHDA- 2002/01/23 10:01
CRDT- 2001/12/06 10:00
PHST- 2001/12/06 10:00 [pubmed]
PHST- 2002/01/23 10:01 [medline]
PHST- 2001/12/06 10:00 [entrez]
AID - S0014-5793(01)03126-X [pii]
AID - 10.1016/s0014-5793(01)03126-x [doi]
PST - ppublish
SO  - FEBS Lett. 2001 Nov 30;509(1):95-100. doi: 10.1016/s0014-5793(01)03126-x.