PMID- 11735217
OWN - NLM
STAT- MEDLINE
DCOM- 20020221
LR  - 20081121
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 78
IP  - 3
DP  - 2001 Dec
TI  - Identification of new regulatory sequences far upstream of the mouse monocyte 
      chemoattractant protein-1 gene.
PG  - 113-23
AB  - We systematically searched for sequences influencing the expression of the mouse 
      monocyte chemoattractant protein-1 (MCP-1) gene (Scya2) by mapping DNase I 
      hypersensitive sites (HS) in the chromatin of mesangial cells in a 40-kb interval 
      around the gene. We found nine HS located between -24 kb and +12.7 kb. Three HS 
      coincided with previously known regulatory sequences (HS-2.4, HS-1.0, and 
      HS-0.2). We tested two of the previously unknown HS located far upstream of Scya2 
      (HS-19.4 and HS-16.3) in transfection experiments using luciferase reporter 
      constructs and mouse mesangial cells as recipients. In transient transfections, 
      both HS had a moderate effect on basal promoter activity as well as promoter 
      activity stimulated by tumor necrosis factor-alpha. In stable transfection 
      experiments, we found much higher activity. A DNA fragment containing HS-19.4 and 
      HS-16.3 caused a considerable increase in the number of stably integrated 
      luciferase copies. We determined the nucleotide sequence of the 5' flanking 
      region to -28.6 kb. Computer-assisted sequence analysis did not yield evidence of 
      an additional gene. These HS are located within the 5' flanking region of a gene 
      cluster consisting of Scya2 (MCP-1), Scya7 (MCP-3), Scya11 (eotaxin), Scya12 
      (MCP-5), and Scya8 (MCP-2). This report represents the first comprehensive 
      chromatin analysis of the mouse MCP-1 locus leading to the identification of a 
      complex regulatory region located far upstream of Scya2.
FAU - Wagner, K
AU  - Wagner K
AD  - Ludwig-Maximilians-Universitat, Medizinische Poliklinik, Schillerstrasse 42, 
      D-80336 Munchen, Germany.
FAU - Dendorfer, U
AU  - Dendorfer U
FAU - Chilla, S
AU  - Chilla S
FAU - Schlondorff, D
AU  - Schlondorff D
FAU - Luckow, B
AU  - Luckow B
LA  - eng
SI  - GENBANK/AJ238892
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chromatin)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.1.21.1 (Deoxyribonuclease I)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*genetics
MH  - Chromatin/metabolism
MH  - Cloning, Molecular
MH  - Deoxyribonuclease I/metabolism
MH  - Glomerular Mesangium/metabolism
MH  - Humans
MH  - Luciferases/genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Promoter Regions, Genetic
MH  - *Regulatory Sequences, Nucleic Acid
MH  - Transfection
EDAT- 2001/12/12 10:00
MHDA- 2002/02/22 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/02/22 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - S0888-7543(01)96660-1 [pii]
AID - 10.1006/geno.2001.6660 [doi]
PST - ppublish
SO  - Genomics. 2001 Dec;78(3):113-23. doi: 10.1006/geno.2001.6660.