PMID- 11736809 OWN - NLM STAT- MEDLINE DCOM- 20020214 LR - 20190916 IS - 0268-9146 (Print) IS - 0268-9146 (Linking) VI - 32 IP - 6 DP - 2001 Dec TI - An updated linkage and comparative map of porcine chromosome 18. PG - 375-9 AB - Swine chromosome 18 (SSC18) has the poorest marker density in the USDA-MARC porcine linkage map. In order to increase the marker density, seven genes from human chromosome 7 (HSA7) expected to map to SSC18 were selected for marker development. The genes selected were: growth hormone releasing hormone receptor (GHRHR), GLI-Kruppel family member (GLI3), leptin (LEP), capping protein muscle Z-line alpha 2 subunit (CAPZA2), beta A inhibin (INHBA), T-cell receptor beta (TCRB) and T-cell receptor gamma (TCRG). Large-insert clones (YACs, BACs and cosmids) that contained these genes, as well as two previously mapped microsatellite markers (SW1808 and SW1984), were identified and screened for microsatellites. New microsatellite markers were developed from these clones and mapped. Selected clones were also physically assigned by fluorescence in situ hybridization (FISH). Fifteen new microsatellite markers were added to the SSC18 linkage map resulting in a map of 28 markers. Six genes have been included into the genetic map improving the resolution of the SSC18 and HSA7 comparative map. Assignment of TCRG to SSC9 has identified a break in conserved synteny between SSC18 and HSA7. FAU - Campbell, E M AU - Campbell EM AD - USDA, ARS, US Meat Animal Research Center, PO Box 166, Spur 18D, Clay Center, NE 68933-0166, USA. FAU - Fahrenkrug, S C AU - Fahrenkrug SC FAU - Vallet, J L AU - Vallet JL FAU - Smith, T P AU - Smith TP FAU - Rohrer, G A AU - Rohrer GA LA - eng PT - Journal Article PL - England TA - Anim Genet JT - Animal genetics JID - 8605704 RN - 0 (CapZ Actin Capping Protein) RN - 0 (DNA-Binding Proteins) RN - 0 (GLI3 protein, Xenopus) RN - 0 (GLI3 protein, human) RN - 0 (Gli3 protein, mouse) RN - 0 (Kruppel-Like Transcription Factors) RN - 0 (Leptin) RN - 0 (Microfilament Proteins) RN - 0 (Muscle Proteins) RN - 0 (Nerve Tissue Proteins) RN - 0 (Receptors, Antigen, T-Cell, alpha-beta) RN - 0 (Receptors, Antigen, T-Cell, gamma-delta) RN - 0 (Repressor Proteins) RN - 0 (Transcription Factors) RN - 0 (Xenopus Proteins) RN - 0 (Zinc Finger Protein Gli3) RN - 0 (inhibin beta A subunit) RN - 93443-12-0 (Inhibin-beta Subunits) SB - IM MH - Animals MH - CapZ Actin Capping Protein MH - Chromosome Mapping/*veterinary MH - Chromosomes, Human, Pair 7 MH - DNA-Binding Proteins/genetics MH - Genetic Linkage MH - Humans MH - In Situ Hybridization, Fluorescence MH - Inhibin-beta Subunits/genetics MH - Kruppel-Like Transcription Factors MH - Leptin/genetics MH - Microfilament Proteins/genetics MH - Microsatellite Repeats/genetics MH - Muscle Proteins/genetics MH - *Nerve Tissue Proteins MH - Receptors, Antigen, T-Cell, alpha-beta/genetics MH - Receptors, Antigen, T-Cell, gamma-delta/genetics MH - *Repressor Proteins MH - Swine/*genetics MH - Transcription Factors/genetics MH - *Xenopus Proteins MH - Zinc Finger Protein Gli3 EDAT- 2001/12/12 10:00 MHDA- 2002/02/15 10:01 CRDT- 2001/12/12 10:00 PHST- 2001/12/12 10:00 [pubmed] PHST- 2002/02/15 10:01 [medline] PHST- 2001/12/12 10:00 [entrez] AID - 782 [pii] AID - 10.1046/j.1365-2052.2001.00782.x [doi] PST - ppublish SO - Anim Genet. 2001 Dec;32(6):375-9. doi: 10.1046/j.1365-2052.2001.00782.x.