PMID- 11736949
OWN - NLM
STAT- MEDLINE
DCOM- 20020107
LR  - 20190705
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 115
IP  - 3
DP  - 2001 Dec
TI  - Correction of stromal cell defect after bone marrow transplantation in aplastic 
      anaemia.
PG  - 642-52
AB  - Defects in stromal cell function have been demonstrated in a number of aplastic 
      anaemia (AA) patients. Here we have studied a patient with severe AA and abnormal 
      stromal cell function who underwent bone marrow transplantation (BMT). The 
      objective of this study was to investigate the timing and the mechanism of 
      correction of the stromal defect after transplantation. The patient, a 
      25-year-old woman with severe AA, underwent BMT from her brother. BM was obtained 
      from the patient on five occasions: 2 weeks pre BMT, and 3, 8, 16 and 21 months 
      post BMT. Stromal cells were grown to confluence and recharged with purified 
      CD34+ cells from normal donors. The support of such cells, as assessed by weekly 
      colony-forming assay (CFU) of non-adherent cells, was compared with that of 
      stromal layers grown from normal BM. A novel technique of combined fluorescence 
      in situ hybridization (FISH) and immunocytochemistry was used to determine the 
      origin of specific stromal cell types on cytospins of stroma post BMT. Stromal 
      function was defective at 2 weeks pre BMT and at 3 months post BMT, but returned 
      to normal at 8 and 16 months post BMT. At 21 months post BMT, stromal fibroblasts 
      and endothelial cells were shown to be of recipient origin, and macrophages and T 
      cells were of donor origin. We present here evidence in a case of severe AA for 
      defective stromal function before BMT and delayed normalization of function after 
      BMT. This correlated with engraftment of donor macrophages and T cells, but not 
      fibroblasts and endothelial cells.
FAU - Scopes, J
AU  - Scopes J
AD  - Department of Haematology, St. George's Hospital Medical School, London, UK.
FAU - Ismail, M
AU  - Ismail M
FAU - Marks, K J
AU  - Marks KJ
FAU - Rutherford, T R
AU  - Rutherford TR
FAU - Draycott, G S
AU  - Draycott GS
FAU - Pocock, C
AU  - Pocock C
FAU - Gordon-Smith, E C
AU  - Gordon-Smith EC
FAU - Gibson, F M
AU  - Gibson FM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
SB  - IM
MH  - Adult
MH  - Anemia, Aplastic/immunology/*therapy
MH  - Bone Marrow Cells/*immunology
MH  - *Bone Marrow Transplantation
MH  - Colony-Forming Units Assay
MH  - Endothelium/immunology
MH  - Female
MH  - Fibroblasts/immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Macrophages/immunology
MH  - Pregnancy
MH  - Pregnancy Complications, Hematologic/immunology/*therapy
MH  - T-Lymphocytes/immunology
MH  - Time Factors
MH  - Transplantation, Homologous
EDAT- 2001/12/12 10:00
MHDA- 2002/01/10 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/01/10 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - 3134 [pii]
AID - 10.1046/j.1365-2141.2001.03134.x [doi]
PST - ppublish
SO  - Br J Haematol. 2001 Dec;115(3):642-52. doi: 10.1046/j.1365-2141.2001.03134.x.