PMID- 11738152
OWN - NLM
STAT- MEDLINE
DCOM- 20020225
LR  - 20190910
IS  - 0531-5565 (Print)
IS  - 0531-5565 (Linking)
VI  - 37
IP  - 1
DP  - 2001 Dec
TI  - CpG DNA functions as an effective adjuvant for the induction of immune responses 
      in aged mice.
PG  - 107-26
AB  - The present studies demonstrate that the immunization of aged mice with 
      Diphtheria toxoid in formulations containing unmethylated immunostimulatory CpG 
      motifs, promotes the successful development of immune responses that are 
      qualitatively and quantitatively comparable to those induced in young animals 
      vaccinated in a similar manner. Aged mice given vaccines containing CpG 
      oligodeoxynucleotides (ODNs) expressed primary and secondary systemic humoral 
      immune responses having isotype profiles consistent with an enhancement in Th-1 
      type immunity. The ability to generate common mucosal immunity was also restored 
      in aged animals given CpG ODN-containing vaccines. Dendritic cells (DCs) were 
      determined to represent one of the cellular targets of CpG ODN activities in aged 
      mice since restoration of immune function was observed when DCs from aged donors 
      were pulsed with antigen and CpG ODNs, prior to injection into syngeneic young 
      adult or aged recipients. Interestingly, antigen-pulsed DCs from young donors 
      were fully capable of stimulating immune responses following their injection into 
      syngeneic young adult or aged hosts, without a need for exposure to CpG ODNs. 
      Although the mechanism(s) by which CpG DNA exerts its beneficial adjuvant effects 
      on the aged immune system remains unclear, our findings suggest that the 
      incorporation of CpG ODNs into vaccine formulations provided to the aged could 
      prove useful in the development of more effective vaccines for the elderly.
FAU - Manning, B M
AU  - Manning BM
AD  - Department of Pathology, University of Utah, 50 N. Medical Drive, Salt Lake City, 
      UT 84132, USA.
FAU - Enioutina, E Y
AU  - Enioutina EY
FAU - Visic, D M
AU  - Visic DM
FAU - Knudson, A D
AU  - Knudson AD
FAU - Daynes, R A
AU  - Daynes RA
LA  - eng
GR  - CA25917/CA/NCI NIH HHS/United States
GR  - DK55491/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, Bacterial)
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (Diphtheria-Tetanus-Pertussis Vaccine)
RN  - 0 (Haemophilus Vaccines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Vaccines, Conjugate)
RN  - 0 (diphtheria-tetanus-pertussis-haemophilus b conjugate vaccine)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - *Adjuvants, Immunologic
MH  - Aging/*immunology
MH  - Animals
MH  - Antibody Formation
MH  - Antigen Presentation/immunology
MH  - Antigens, Bacterial/immunology
MH  - Bone Marrow Cells/cytology/immunology
MH  - Calcitriol/immunology
MH  - CpG Islands/*immunology
MH  - Dendritic Cells/immunology
MH  - Diphtheria-Tetanus-Pertussis Vaccine/immunology
MH  - Female
MH  - Haemophilus Vaccines/immunology
MH  - Immunoglobulin G/biosynthesis
MH  - Immunophenotyping
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred DBA
MH  - Oligodeoxyribonucleotides/*immunology
MH  - Th1 Cells/immunology
MH  - Vaccines, Conjugate/immunology
EDAT- 2001/12/12 10:00
MHDA- 2002/02/28 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/02/28 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - S0531-5565(01)00157-7 [pii]
AID - 10.1016/s0531-5565(01)00157-7 [doi]
PST - ppublish
SO  - Exp Gerontol. 2001 Dec;37(1):107-26. doi: 10.1016/s0531-5565(01)00157-7.