PMID- 11738649
OWN - NLM
STAT- MEDLINE
DCOM- 20020212
LR  - 20231213
IS  - 0034-5687 (Print)
IS  - 0034-5687 (Linking)
VI  - 129
IP  - 1-2
DP  - 2001 Dec
TI  - Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive 
      brainstem regions in rat.
PG  - 101-21
AB  - Recent studies have suggested that cell-to-cell coupling, which occurs via gap 
      junctions, may play a role in CO(2) chemoreception. Here, we used immunoblot and 
      immunohistochemical analyses to investigate the presence, distribution, and 
      cellular localization of the gap junction proteins connexin26 (Cx26) and 
      connexin32 (Cx32) in putative CO(2)-chemosensitive brainstem regions in both 
      neonatal and adult rats. Immunoblot analyses revealed that both Cx subtypes were 
      expressed in putative CO(2)-chemosensitive brainstem regions; however, regional 
      differences in expression were observed. Immunohistochemical experiments 
      confirmed Cx expression in each of the putative CO(2)-chemosensitive brainstem 
      regions, and further demonstrated that Cx26 and Cx32 were found in neurons and 
      Cx26 was also found in astrocytes in these regions. Thus, our findings suggest 
      the potential for gap junctional communication in these regions in both neonatal 
      and adult rats. We propose that the gap junction proteins Cx26 and Cx32, at least 
      in part, form the neuroanatomical substrate for this gap junctional 
      communication, which is hypothesized to play a role in central CO(2) 
      chemoreception.
FAU - Solomon, I C
AU  - Solomon IC
AD  - Department of Physiology and Biophysics, State University of New York, Basic 
      Science Tower, Health SC. Center, Level 6, Rm. 140, Stony Brook, NY 11794-8661, 
      USA. icsolomon@physiology.pnb.sunysb.edu
FAU - Halat, T J
AU  - Halat TJ
FAU - El-Maghrabi, M R
AU  - El-Maghrabi MR
FAU - O'Neal, M H 3rd
AU  - O'Neal MH 3rd
LA  - eng
GR  - HL63175/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Respir Physiol
JT  - Respiration physiology
JID - 0047142
RN  - 0 (Connexins)
RN  - 0 (Gjb2 protein, rat)
RN  - 127120-53-0 (Connexin 26)
RN  - 142M471B3J (Carbon Dioxide)
SB  - IM
MH  - Animals
MH  - Astrocytes/metabolism
MH  - Brain Stem/cytology/metabolism/*physiology
MH  - Carbon Dioxide/*metabolism
MH  - Chemoreceptor Cells/*physiology
MH  - Connexin 26
MH  - Connexins/*metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Neurons/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tissue Distribution
MH  - Gap Junction beta-1 Protein
EDAT- 2001/12/12 10:00
MHDA- 2002/02/13 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/02/13 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - S0034568701002997 [pii]
AID - 10.1016/s0034-5687(01)00299-7 [doi]
PST - ppublish
SO  - Respir Physiol. 2001 Dec;129(1-2):101-21. doi: 10.1016/s0034-5687(01)00299-7.