PMID- 11738826
OWN - NLM
STAT- MEDLINE
DCOM- 20020228
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 280
IP  - 1-2
DP  - 2001 Dec 12
TI  - Identification of a novel family of putative methyltransferases that interact 
      with human and Drosophila presenilins.
PG  - 135-44
AB  - Mutations in the presenilin genes have been shown to cause the majority of cases 
      of early-onset familial Alzheimer's disease (AD). In addition to their role in 
      AD, presenilins are also known to function during development by interacting with 
      the Notch pathway. To determine if presenilins have additional functions during 
      development and AD we have used a yeast two-hybrid approach to search for 
      proteins that can bind to presenilins. Here, we show the identification and 
      characterization of a novel putative methyltransferase (Metl) that interacts with 
      the loop region of Drosophila presenilin as well as human presenilin-1 and 
      presenilin-2, suggesting that this interaction is evolutionarily conserved and 
      functionally important. Metl appears to be a member of a conserved family of 
      methyltransferases that share homology with, but are distinct from, the UbiE 
      family of methyltransferases involved in ubiquinone and menaquinone biosynthesis. 
      In Drosophila, the metl gene gives rise to two major isoforms by alternative 
      splicing that are broadly expressed throughout development and found in the 
      central nervous system in an overlapping pattern with Drosophila presenilin. 
      Finally, we show that two independent dominant adult phenotypes produced by 
      overexpression of presenilin can be enhanced by overexpression of metl in the 
      same tissue. Taken together, these results suggest that presenilin and Metl 
      functionally and genetically interact during development.
FAU - Zhang, S X
AU  - Zhang SX
AD  - Program in Developmental Biology, The Hospital for Sick Children, 555 University 
      Avenue, Ontario, Toronto, Canada.
FAU - Guo, Y
AU  - Guo Y
FAU - Boulianne, G L
AU  - Boulianne GL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Isoenzymes)
RN  - 0 (Membrane Proteins)
RN  - 0 (PSEN1 protein, human)
RN  - 0 (PSEN2 protein, human)
RN  - 0 (Presenilin-1)
RN  - 0 (Presenilin-2)
RN  - 0 (RNA, Messenger)
RN  - EC 2.1.1.- (Methyltransferases)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Drosophila/embryology/genetics/growth & development
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Gene Expression Regulation, Enzymologic
MH  - Genes/genetics
MH  - Humans
MH  - Isoenzymes/genetics/metabolism
MH  - Male
MH  - Membrane Proteins/genetics/*metabolism
MH  - Methyltransferases/*genetics/metabolism
MH  - Molecular Sequence Data
MH  - Presenilin-1
MH  - Presenilin-2
MH  - Protein Binding
MH  - RNA, Messenger/genetics/metabolism
MH  - Saccharomyces cerevisiae/genetics
MH  - Sequence Homology, Amino Acid
MH  - Two-Hybrid System Techniques
EDAT- 2001/12/12 10:00
MHDA- 2002/03/01 10:01
CRDT- 2001/12/12 10:00
PHST- 2001/12/12 10:00 [pubmed]
PHST- 2002/03/01 10:01 [medline]
PHST- 2001/12/12 10:00 [entrez]
AID - S0378-1119(01)00770-3 [pii]
AID - 10.1016/s0378-1119(01)00770-3 [doi]
PST - ppublish
SO  - Gene. 2001 Dec 12;280(1-2):135-44. doi: 10.1016/s0378-1119(01)00770-3.